NAME: Li, Chunman

POSITION TITLE: Assistant Professor; Department of Anatomy, Histology and Development Biology, Shenzhen University Health Science Center, Shenzhen, China

EDUCATION/TRAINING

A.   Personal Statement

My research interests lie in the molecular mechanisms of membrane trafficking and lipid metabolism. My r esearch in lipid metabolism has been supported by the General Program of National Natural Science Foundation in China (NSFC) , 2018 . I have published 19 papers in professional, peer-reviewed journals .

B.   Positions and Honors

Positions and Employment

08/1996–08/1999        Resident Surgeon, Department of General Surgery, Tianjin 1^st Central Hospital, Tianjin, China

11/2016 – present      Assistant Professor, Department of Anatomy, Histology and Development Biology, Shenzhen University Health Science Center, Shenzhen, China

C.   Contributions to Science (*: co rresponding author)

A full list of my publications (in a total of 74) is enclosed at the end of this document.

1.    Regulation of anterograde trafficking of GPCRs

G-protein-coupled receptors (GPCRs) constitute the largest super-family of cell-surface signaling proteins. However, the mechanisms underlying their surface targeting and sorting are poorly understood. During my postdoctoral position in Dr Guangyu Wu’s lab, I determined the role of Rab26 (a Ras-like small GTPase involved in vesicle-mediated secretion) in the cell surface export of α(2)-adrenergic receptors (JBC 2012, 2003). I also found that manipulation of Rab43 function significantly alters the surface presentation and signaling of all GPCRs studied without affecting non-GPCR membrane proteins. This work was published in 2017 (Cell Reports, 2017).

1)    Li C, Fan Y, Lan TH, Lambert NA, Wu G*. 2012. Rab26 Modulates the Cell-Surface Transport of α2-Adrenergic Receptors from the Golgi. J Biol Chem. 287(51):42784-94.

2)    Li C, Wei Z, Fan Y, Huang W, Su Y, Li H, Dong Z, Fukuda M, Khater M, Wu G*. 2017. The GTPase Rab43 Controls the Anterograde ER-Golgi Trafficking and Sorting of GPCRs. Cell Rep. 21(4):1089-1101

2.    Lipid droplet formation and lipolysis

Lipid droplets (LDs) are highly dynamic organelles that not only store neutral lipids but also are involved in multiple cellular processes. Dysregulation of lipogenesis or lipolysis greatly contributes to the pathogenesis of several human diseases, including obesity, diabetes, and fatty liver disease. During my time as a research associate in Dr. Sidney Yu’s lab, I discovered a COPI–TRAPPII–Rab18 signaling mechanism in LD homeostasis. The related work was published in 2017 (EMBO J, 2017).

1)    Li C, Luo X, Zhao S, Siu GK, Liang Y, Chan HC, Satoh A, Yu SS*. 2017.COPI-TRAPPII activates Rab18 and regulates its lipid droplet association.EMBO J. 36(4):441-457.

D.   Research Support

Ongoing Research Support

NSFC (China)  (31771567)                   01/01/2018 to 12/31/2021

Chunman Li, PI                                      ￥570,000 (direct cost)

Study on the mechanism of transporter protein complex II (TRAPP II) and small G protein Rab18 in the regulation of lipid droplets in mammalian cells and the pathogenesis of hepatic fibrosis

E.    Peer-reviewed publications ( # : co -first author)

(1)     Chunman Li, Zhe Wei, Yi Fan, Wei Huang, Yunchao Su, Honglin Li, Zheng Dong, Mitsunori Fukuda, Mostafa Khater, Guangyu Wu*. The GTPase Rab43 controls the anterograde ER-Golgi trafficking and sorting of GPCRs. Cell Reperts, 2017, 21(4):1089-1101.

(2)     Huju Chi#, Chunman Li#, Flora Sha Zhao, Li Zhang, Tzi Bun Ng*, Guangyi Jin*, Ou Sha*. Anti-tumor Activity of Toll-Like Receptor 7 Agonists. Frontiers in Pharmacology, 2017, 8:304.

(3) Chunman Li, Meiqi Zeng, Huju Chi, Jing Shen, Tzi-Bun Ng, Guangyi Jin, Desheng Lu, Xinmin Fan, Bilian Xiong, Zhangang Xiao*, Ou Sha*. Trichosanthin increases Granzyme B penetration into tumor cells by upregulation of CT-MPR on the cell surface. Oncotarget, 2017, 8(16):26460-26470

(4) Chunman Li, Xiaomin Luo, Shan Zhao, Gavin KY Siu, Yongheng Liang, Hsiao Chang Chan, Ayano Satoh, Sidney SB Yu*. COPI–TRAPPII activates Rab18 and regulates its lipid droplet association, The EMBO Journal, 2017, 36, 441 ~ 457

(5) Chunman Li, Sidney S. B. Yu*. Rab proteins as regulators of lipid droplet formation and lipolysis, Cell Biology International, 2016, 40(10): 1026 ~ 1032

(6) Junfei Niu#, Chunman Li#, Haihui Wu, Xianling Feng, Qingning Su, Shihe Li, Lihong Zhang, David Tai Wai Yew, Eric Yu Pang Cho*, Ou Sha*. Propidium iodide (PI) stains Nissl bodies and may serve as a quick marker for total neuronal cell count, Acta Histochemica,2015, 117(2): 182 ~ 187

(7) Chunman Li*, Sidney Yu. A perspective from transport protein particle: Vesicle tether and human diseases, Acta Physiologica Sinica, 2014, 66(1): 1 ~ 6

(8) Chunman Li, Yi Fan, Tien-Hung Lan, Nevin A. Lambert, Guangyu Wu*. Rab26 Modulates the Cell Surface Transport of α2-Adrenergic Receptors from the Golgi, The Journal of Biological Chemistry, 2012, 287(51): 42784 ~ 42794

(9) Yan Wang, Wei Qi, Li Zhang, Zhenguang Ying,4Ou Sha,Chunman Li, Lanhai Lü, Xiangyan Chen,Zhenzhong Li, Feng Niu, Fang Xue,Dong Wang, Tzi-Bun Ng, and Lihong Zhang*. The novel targets of DL-3-n-butylphthalide predicted by similarity ensemble approach in combination with molecular docking study.Quant Imaging Med Surg. 2017 Oct;7(5):532-536.

(10)Xiaomin Luo,   Chunman Li,   Ran Tan,   Xiaohui Xu,   William K.K. Wu,   Ayano Satoh,   Tuanlao Wang,   Sidney Yu*. A RasGAP, DAB2IP, regulates lipid droplet homeostasis by serving as GAP toward RAB40C. Oncotarget. 2017; 8:85415-85427.

(11) Shan Zhao, Chun Man Li, Xiao Min Luo, Gavin Ka Yu Siu, Wen Jia Gan, Lin Zhang, William K. K. Wu, Hsiao Chang Chan,  and Sidney Yu*.Mammalian TRAPPIII Complex positively modulates the recruitment of Sec13/31 onto COPII vesicles. Sci Rep. 2017; 7: 43207.

(12) Maoxiang Zhang, Jason E. Davis, Chunman Li, Jie Gao, Wei Huang, Nevin A. Lambert, Alvin V. Terry, Jr., Guangyu Wu*. GGA3 Interacts with a G Protein-Coupled Receptor and Modulates Its Cell Surface Export, Molecular and Cellular Biology, 2016, 36(7): 1152 ~ 1163

(13) Tien-Hung Lan, Qiuju Liu, Chunman Li, Guangyu Wu, Jan Steyaert, Nevin A. Lambert*. BRET evidence that β2 adrenergic receptors do not oligomerize in cells, Scientific Reports, 2015, 8(5): 10166

(14) Yi Fan, Chunman Li, Jianhui Guo, Gang Hu, Guangyu Wu*. A Single Lys Residue on the First Intracellular Loop Modulates the Endoplasmic Reticulum Export and Cell-Surface Expression of α2A-Adrenergic Receptor, PLoS One, 7(12): e50416

(15) Tien-Hung Lan, Qiuju Liu, Chunman Li, Guangyu Wu, Nevin A. Lambert*. Sensitive and High Resolution Localization and Tracking of Membrane Proteins in Live Cells with BRET, Traffic, 2012, 13(11):1450 ~ 1456

(16) Chunmin Dong, Chunman Li, Guangyu Wu*. Regulation of α2B-Adrenergic Receptor-mediated Extracellular Signal-regulated Kinase 1/2 (ERK1/2) Activation by ADP-ribosylation Factor 1, The Journal of Biological Chemistry, 2011, 286(50): 43361 ~ 43369

(17) Florence W. K. Cheung, Chunman Li, Chun-Tao Che, Bonnie P. L. Liu, Lijun Wang, Wing-Keung Liu*. Geoditin A Induces Oxidative Stress and Apoptosis on Human Colon HT29 Cells, Marine Drugs, 2010, 8(1): 80 ~ 90

(18) Ning Kang, Lian Duan, Long Tang, Shirong Liu, Chunman Li, Yonghai Li, Qingfeng Liu, Yu Hu, Lianxian Cui, Wei He*. Identification and Characterization of a Novel Thymus Aging Related Protein Rwdd1, Cellular & Molecular Immunology, 2008, 5(4): 279 ~ 285

(19) Wing-keung Liu*, Florence W. K. Cheung, Bonnie P. L. Liu, Chunman Li, Wencai Ye, Chun-Tao Che. Involvement of p21 and FasL in induction of cell cycle arrest and apoptosis by neochamaejasmin A in human prostate LNCaP cancer cells, Journal of Natural Products, 2008, 71(5): 842 ~ 846