NAME: Xu, Jianyong

POSITION TITLE: Assistant Professor of Immunology,, Shenzhen University School of Medicine

EDUCATION/TRAINING

A.   Personal Statement

My research interests are stem cell biology, genome editing and their therapeutic applications. During 2005 to 2008, I worked in the Yellow Sea Fishery Research Institute, Chinese Academy of Fishery Sciences as master student. My main projects include gene cloning and expression profiling of genes associated with pathogen resistance, muscle development and sex differentiation; genetic markers (SSR, RFLP, SNP) development for breeding; characterization of fish bacteria; BAC (bacterial artificial chromosome) library construction and positioning cloning; SNP association analysis of genes related to resistance to pathogen in fish. From 2008 to 2012, I worked in the Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences as research assistant. My projects include the role of HIF (hypoxia inducible factor) pathway in renal cancer development; cilia regulation via HIF pathway; HIF targets screening via high throughput sequencing (RNA-seq, miRNA-seq and ChIP-seq); MicroRNA regulation by HIF; the role of vitamin C in treatment of renal cancer; mouse, pig and human iPSC (induced pluripotent stem cell) generation and characterization; the role of hypoxia in pluripotency maintenance; target gene knockout in human pluripotent stem cells with ZFN (zinc finger nuclease) and Talen. From 2012 to 2016, I studied in the University of Hong Kong as PhD student. My projects include target gene knockout and correction in human pluripotent stem cells with Talen and Cas9; cardiomyocytes differentiation from pluripotent stem cells; cardiomyocytes characterization by calcium transient, action potential analysis; cardiac progenitor cells in cardiac regeneration after myocardial infarction; induced cardiac progenitor cells from skin fibroblasts through somatic reprogramming; humanized mice construction with human CD34+ HSC from cord blood. From 2017, I am working in SZU, my projects mainly focus on the mesenchymal stem cell biology and their therapeutic applications.

B.   Contributions to Science (*: corresponding/co-corresponding author)

C.   Research Support

Science Foundation of SZU                   06/01/2017 to 5/31/2019

Jianyong Xu, PI                                                           ￥30,000

Therapeutic application of MSC in treating SLE mice model

Science Foundation of Shenzhen                 06/01/2017 to 5/31/2019

Jianyong Xu, PI                                                           ￥300,000

Optimized strategy to improve the immune regulation effects of MSC

Starting Fund to Jianyong Xu                 06/01/2017 to 5/31/2020

Jianyong Xu, PI                                                           ￥2700,000

Therapeutic application and manipulation of MSC

Medical Foundation of Guangdong       06/01/2018 to 5/31/2020

Jianyong Xu, PI                                                           ￥10,000

Generation of cardiac progenitor cells via somatic reprogramming

D.   Peer-reviewed publications (*: corresponding author)

1     Xu J: Therapeutic Applications of Mesenchymal Stem Cells for Systemic Lupus Erythematosus. Adv Exp Med Biol 2018;10.1007/5584_2018_212

2     Xu J, Lian W, Li L, Huang Z: Generation of induced cardiac progenitor cells via somatic reprogramming. Oncotarget 2017;8:29442-29457.

3     Xu J, Lian W, Jia Y, Li L, Huang Z: Optimized guide RNA structure for genome editing via Cas9. Oncotarget 2017;8:94166-94171.

4     Xu JY, Lee YK, Ran X, Liao SY, Yang J, Au KW, Lai WH, Esteban MA, Tse HF: Generation of Induced Cardiospheres via Reprogramming of Skin Fibroblasts for Myocardial Regeneration. Stem Cells 2016;10.1002/stem.2438

5     Xu JY, Lee YK, Wang Y, Tse HF: Therapeutic application of endothelial progenitor cells for treatment of cardiovascular diseases. Curr Stem Cell Res Ther 2014;9:401-414.

6     Yao Y, Nashun B, Zhou T, Qin L, Qin L, Zhao S, Xu J, Esteban MA, Chen X: Generation of CD34+ cells from CCR5-disrupted human embryonic and induced pluripotent stem cells. Hum Gene Ther 2012;23:238-242.

7     Xu J, Wang B, Xu Y, Sun L, Tian W, Shukla D, Barod R, Grillari J, Grillari-Voglauer R, Maxwell PH, Esteban MA: Epigenetic regulation of HIF-1alpha in renal cancer cells involves HIF-1alpha/2alpha binding to a reverse hypoxia-response element. Oncogene 2012;31:1065-1072.

8     Zhang S, Chen S, Li W, Guo X, Zhao P, Xu J, Chen Y, Pan Q, Liu X, Zychlinski D, Lu H, Tortorella MD, Schambach A, Wang Y, Pei D, Esteban MA: Rescue of ATP7B function in hepatocyte-like cells from Wilson's disease induced pluripotent stem cells using gene therapy or the chaperone drug curcumin. Hum Mol Genet 2011;20:3176-3187.

9     Ho JC, Zhou T, Lai WH, Huang Y, Chan YC, Li X, Wong NL, Li Y, Au KW, Guo D, Xu J, Siu CW, Pei D, Tse HF, Esteban MA: Generation of induced pluripotent stem cell lines from 3 distinct laminopathies bearing heterogeneous mutations in lamin A/C. Aging (Albany NY) 2011;3:380-390.

10   Yang J, Cai J, Zhang Y, Wang X, Li W, Xu J, Li F, Guo X, Deng K, Zhong M, Chen Y, Lai L, Pei D, Esteban MA: Induced pluripotent stem cells can be used to model the genomic imprinting disorder Prader-Willi syndrome. J Biol Chem 2010;285:40303-40311.

11   Xu J, Li H, Wang B, Xu Y, Yang J, Zhang X, Harten SK, Shukla D, Maxwell PH, Pei D, Esteban MA: VHL inactivation induces HEF1 and Aurora kinase A. J Am Soc Nephrol 2010;21:2041-2046.

12   Shao CW, Chen SL, Scheuring CF, Xu JY, Sha ZX, Dong XL, Zhang HB: Construction of two BAC libraries from half-smooth tongue sole Cynoglossus semilaevis and identification of clones containing candidate sex-determination genes. Mar Biotechnol (NY) 2010;12:558-568.

13   Pei D, Xu J, Zhuang Q, Tse HF, Esteban MA: Induced pluripotent stem cell technology in regenerative medicine and biology. Adv Biochem Eng Biotechnol 2010;123:127-141.

14   Li R, Liang J, Ni S, Zhou T, Qing X, Li H, He W, Chen J, Li F, Zhuang Q, Qin B, Xu J, Li W, Yang J, Gan Y, Qin D, Feng S, Song H, Yang D, Zhang B, Zeng L, Lai L, Esteban MA, Pei D: A mesenchymal-to-epithelial transition initiates and is required for the nuclear reprogramming of mouse fibroblasts. Cell Stem Cell 2010;7:51-63.

15   Esteban MA, Wang T, Qin B, Yang J, Qin D, Cai J, Li W, Weng Z, Chen J, Ni S, Chen K, Li Y, Liu X, Xu J, Zhang S, Li F, He W, Labuda K, Song Y, Peterbauer A, Wolbank S, Redl H, Zhong M, Cai D, Zeng L, Pei D: Vitamin C enhances the generation of mouse and human induced pluripotent stem cells. Cell Stem Cell 2010;6:71-79.

16   Esteban MA, Peng M, Deli Z, Cai J, Yang J, Xu J, Lai L, Pei D: Porcine induced pluripotent stem cells may bridge the gap between mouse and human iPS. IUBMB Life 2010;62:277-282.

17   Chen J, Liu J, Han Q, Qin D, Xu J, Chen Y, Yang J, Song H, Yang D, Peng M, He W, Li R, Wang H, Gan Y, Ding K, Zeng L, Lai L, Esteban MA, Pei D: Towards an optimized culture medium for the generation of mouse induced pluripotent stem cells. J Biol Chem 2010;285:31066-31072.

18   Cai J, Li W, Su H, Qin D, Yang J, Zhu F, Xu J, He W, Guo X, Labuda K, Peterbauer A, Wolbank S, Zhong M, Li Z, Wu W, So KF, Redl H, Zeng L, Esteban MA, Pei D: Generation of human induced pluripotent stem cells from umbilical cord matrix and amniotic membrane mesenchymal cells. J Biol Chem 2010;285:11227-11234.

19   Xu JY, Xu GB, Chen SL: A new method for SNP discovery. Biotechniques 2009;46:201-208.

20  Xu JY, Chen SL, Ding H: Specific MHC class II B alleles associated with resistance to Edwardsiella tarda in turbot, Psetta maxima (L.). J Fish Dis 2009;32:637-640.

21   Esteban MA, Xu J, Yang J, Peng M, Qin D, Li W, Jiang Z, Chen J, Deng K, Zhong M, Cai J, Lai L, Pei D: Generation of induced pluripotent stem cell lines from Tibetan miniature pig. J Biol Chem 2009;284:17634-17640.

22   Chen SL, Tian YS, Yang JF, Shao CW, Ji XS, Zhai JM, Liao XL, Zhuang ZM, Su PZ, Xu JY, Sha ZX, Wu PF, Wang N: Artificial gynogenesis and sex determination in half-smooth tongue sole (Cynoglossus semilaevis). Mar Biotechnol (NY) 2009;11:243-251.

23   Qin D, Gan Y, Shao K, Wang H, Li W, Wang T, He W, Xu J, Zhang Y, Kou Z, Zeng L, Sheng G, Esteban MA, Gao S, Pei D: Mouse meningiocytes express Sox2 and yield high efficiency of chimeras after nuclear reprogramming with exogenous factors. J Biol Chem 2008;283:33730-33735.

24   Ye HQ, Chen SL, Xu JY: Molecular cloning and characterization of the Myf5 gene in sea perch (Lateolabrax japonicus). Comp Biochem Physiol B Biochem Mol Biol 2007;147:452-459.

25   Chen SL, Zhang YX, Xu JY, Meng L, Sha ZX, Ren GC: Molecular cloning, characterization and expression analysis of natural resistance associated macrophage protein (Nramp) cDNA from turbot (Scophthalmus maximus). Comp Biochem Physiol B Biochem Mol Biol 2007;147:29-37.

26   Chen SL, Li J, Deng SP, Tian YS, Wang QY, Zhuang ZM, Sha ZX, Xu JY: Isolation of female-specific AFLP markers and molecular identification of genetic sex in half-smooth tongue sole (Cynoglossus semilaevis). Mar Biotechnol (NY) 2007;9:273-280.