NAME: Xia, Lixin

POSITION TITLE: Professor of biochemistry, Shenzhen University School of Medicine

EDUCATION/TRAINING

INSTITUTION AND LOCATION	DEGREE	Completion Date MM/YYYY	FIELD OF STUDY
Nankai University, China	BS	07/1998	Biochemistry
Nankai University, China	MS	07/2001	Genetics
The Chinese University of China, Hongkong, China	PhD	09/2004	Biochemistry
Florida State University	Postdoctoral	03/2009	Immunology

 

A.   Personal Statement

My long-standing research interests fall in two areas:

1) allergic diseases

Here, my lab is actively exploring novel allergens and new mechanism of allergic diseases by investigating new biomarkers such as miRNAs and lncRNAs.

 

2) bio-active products and synthesized chemical compounds for tumor therapy

I have isolated dozens of proteins with antitumor activities. At the same I have explored the antitumor activity of cisplatin derivatives by specifically targeting telomerase-negative cancers.

 

Research in my lab has been continuously supported by the National Natural Science Foundation in China (NSFC) with two regular grants and by Shenzhen Science and Technology Innovation Committee with two consecutive grants. I have published > 30 papers in professional, peer-reviewed journals. I have mentor ed three postdoctoral fellows, two MSc graduates and three MSc candidates.

 

B.   Positions and Honors

Positions and Employment

10 / 2004 – 06 / 2007       Assistant Professor and Associate Professor, College of Life Science, Shenzhen University, China

03 / 2004 – present        Associate Professor and Professor, College of Life Science, Shenzhen University, China

 

Other Experience and Honors

Editorial board, Frontiers in Microbiology (IF: 4.076 ): 2016 -present

 

C.   Contributions to Science (*: corresponding/co-corresponding author)

A full list of my publications (in a total of 74) is enclosed at the end of this document.

 

1. I discovered a new allergen from mango and named it according to international nomenclature, and discovered and characterized a new allergen from cockroach

 

1)    Chen JJ, Xia L, Wu XL, Yang SJ, Ji KM, Liu ZG, Tang MJ, Gao C. A practical test system for sensitive, rapid screening and authentication of peanut allergens in imported and exported food products in Chinese Customs. Food Control. 2012;23(1):154-8.

 

2)    Ji KM, Chen JJ, Gao C, Liu XY, Xia L*, Liu ZG, Li L, Yang SH. A two-site monoclonal antibody immunochromatography assay for rapid detection of peanut allergen Ara h1 in Chinese imported and exported foods. Food chemistry. 2011;129(2):541-5.TOP

 

3)    Xia L, Willison LN, Porter L, Robotham JM, Teuber SS, Sathe SK, Roux KH. Mapping of a conformational epitope on the cashew allergen Ana o 2: a discontinuous large subunit epitope dependent upon homologous or heterologous small subunit association. Mol Immunol. 2010;47(9):1808-16.

 

4)       Robotham JM, Xia L, Willison LN, Teuber SS, Sathe SK, Roux KH. Characterization of a cashew allergen, 11S globulin (Ana o 2), conformational epitope. Mol Immunol. 2010;47(9):1830-8.

 

2. Identified bio-active products and synthesized chemical compounds for tumor therapy, and discovered new types of antifungal proteins, many of which are from edible sources (e.g. beans), including nonspecific lipid transfer proteins, allergen-like peptides. Previously reported types of antifungal proteins including thaumatin-like proteins, defensins and chitinases were also found.

 

1)    Chan YS, Cheung RC, Xia L*, Wong JH, Ng TB, Chan WY. Snake venom toxins: toxicity and medicinal applications. Appl Microbiol Biotechnol. 2016 Jul;100(14):6165-81. doi: 10.1007/s00253-016-7610-9. Epub 2016 Jun 1.

 

2)    Chan YS, Xia L*, Ng TB. White kidney bean lectin exerts anti-proliferative and apoptotic effects on cancer cells. Int J Biol Macromol. 2016 Apr;85:335-45.

 

3)    Lin P, Xia L, Ng TB. First isolation of an antifungal lipid transfer peptide from seeds of a Brassica species. Peptides. 2007;28:1514-9.

 

4)       Xia L, Ng TB. A hemagglutinin with mitogenic activity from dark red kidney beans. J Chromatogr B Analyt Technol Biomed Life Sci. 2006;844:213-6.

 

3. Synthesized a patented cisplatin derivative Tetra-Pt(bpy) that inhibits the strand invasion/annealing step of telomeric homologous recombination by selectively converting telomeric ssDNA to a G-quadruplex in telomerase negative cancer cells and murine model. Importantly, Tetra-Pt(bpy) exhibits no adverse effects on proliferation, gene expression, or telomere metabolism in normal cells.

 

1)    Zheng XH, Nie X, Fang Y, Zhang Z, Xiao Y, Mao Z, Liu H, Ren J, Wang F, Xia L*, Huang J, Zhao Y. J. A Cisplatin Derivative Tetra-Pt(bpy) as an Oncotherapeutic Agent for Targeting ALT Cancer. Natl Cancer Inst. 2017 Oct 1;109(10). doi: 10.1093/jnci/djx061.

 

2)    Chen MZ, Liu XY, Du J, Wang XJ, Xia L*. Differentiated regulation of immune-response related genes between LUAD and LUSC subtypes of lung cancers. Oncotarget. 2016 Nov 15. doi: 10.18632/oncotarget.13346

 

3) Zheng XH, Nie X, Liu HY, Fang YM, Zhao Y, Xia L*. TMPyP4 promotes cancer cell migration at low doses, but induces cell death at high doses. Sci Rep. 2016 May 25;6:26592. doi: 10.1038/srep26592.

 

D.   Research Support

Ongoing Research Support

 

Lixin Xia, PI                                                                                       ￥500,000

New compounds targeting telomerase negative tumor

Science and technology foundation of Shenzhen city              04/01/2018 to 03/31/2020

 

Completed Research Support

 

Lixin Xia, PI                                                                                       ￥320,000

Proteomics of peanut allergen, Ara h 2

NSFC (China)                                                                                          01/01/2009 to 12/31/2011

 

Lixin Xia, PI                                                                                       ￥600,000

Allergenicity and of 30 kD lipoprotein family from silkworm and mechanism of silkworm allergies

NSFC (China)                                                                                          01/01/2013 to 12/31/2016

 

Lixin Xia, PI                                                                                       ￥50,000

MiRNA profile in intestines of food allergy mouse

Science and technology foundation of Guangdong Pr.            10/01/2015 to 09/30/2017

 

Lixin Xia, PI                                                                                       ￥300,000

Applied fundamental research of platinum complexes as telomerase negative tumor chemotherapy drugs

Science and technology foundation of Shenzhen city              10/01/2015 to 09/30/2017

 

 

E.    Peer-reviewed publications (*: corresponding author)

1.      Zheng XH, Nie X, Fang Y, Zhang Z, Xiao Y, Mao Z, Liu H, Ren J, Wang F, Xia L*, Huang J, Zhao Y. J. A Cisplatin Derivative Tetra-Pt(bpy) as an Oncotherapeutic Agent for Targeting ALT Cancer. Natl Cancer Inst. 2017 Oct 1;109(10). doi: 10.1093/jnci/djx061.

2.      Chen MZ, Liu XY, Du J, Wang XJ, Xia L*. Differentiated regulation of immune-response related genes between LUAD and LUSC subtypes of lung cancers. Oncotarget. 2016 Nov 15. doi: 10.18632/oncotarget.13346

3.      Zheng XH, Nie X, Liu HY, Fang YM, Zhao Y, Xia L*. TMPyP4 promotes cancer cell migration at low doses, but induces cell death at high doses. Sci Rep. 2016 May 25;6:26592. doi: 10.1038/srep26592.

4.      Chan YS, Cheung RC, Xia L*, Wong JH, Ng TB, Chan WY. Snake venom toxins: toxicity and medicinal applications. Appl Microbiol Biotechnol. 2016 Jul;100(14):6165-81. doi: 10.1007/s00253-016-7610-9. Epub 2016 Jun 1.

5.      Ng TB, Cheung RC, Wong JH, Chan YS, Dan X, Pan W, Wang H, Guan S, Chan K, Ye X, Liu F, Xia L, Chan WY. Fungal proteinaceous compounds with multiple biological activities. Appl Microbiol Biotechnol. 2016 Jun 23. Chan YS, Xia L*, Ng TB. White kidney bean lectin exerts anti-proliferative and apoptotic effects on cancer cells. Int J Biol Macromol. 2016 Apr;85:335-45.

6.      Chan YS, Yu H, Xia L, Ng TB. Lectin from green speckled lentil seeds (Lens culinaris) triggered apoptosis in nasopharyngeal carcinoma cell lines. Chin Med. 2015 Sep 8;10:25

7.      Chan YS, Xia L*, Ng TB. A Glucosamine-Specific Lectin from Green Dragon No. 8 Beans (Phaseolus vulgaris) Induced Apoptosis on Nasopharyngeal Carcinoma Cells. Evid Based Complement Alternat Med. 2015;2015:760539

8.      Wong JH, Liu Z, Law KW, Liu F, Xia L, Wan DC, Ng TB. A study of effects of peptide fragments of bovine and human lactoferrins on activities of three key HIV-1 enzymes.

9.      Peptides. 2014 Dec;62:183-8.

10.   He W, Yang C, Xia L, Zhao MZ, Ge RT, Huang H, Ji Q, Yang PC, Liu Z. CD4(+) T cells from food allergy model are resistant to TCR-dependent apoptotic induction. Cytokine. 2014 Jul;68(1):32-9. doi: 10.1016/j.cyto.2014.03.010. Epub 2014 Apr 16.

11.   Yan H, Yi H, Xia L, Zhan Z, He W, Cao J, Yang PC, Liu Z. Staphylococcal enterotoxin B suppresses Alix and compromises intestinal epithelial barrier functions. J Biomed Sci. 2014 Apr 9;21:29. doi: 10.1186/1423-0127-21-29.

12.   Liu X, Xiao X, Xia L, Yang PC, Liu Z, Li L. Identification and characterization of a panallergen profilin, a new allergen from Caryota mitis pollen. Asia Pac J Allergy Immunol. 2014;32:40-6.

13.   Chen JJ, Xia L, Wu XL, Yang SJ, Ji KM, Liu ZG, Tang MJ, Gao C. A practical test system for sensitive, rapid screening and authentication of peanut allergens in imported and exported food products in Chinese Customs. Food Control. 2012;23(1):154-8.

14.   Wu XL, Wang WP, Xia L, Xu H, Wu H, Liu ZG. In vitro refolding process of bovine allergen β-lactoglobulin by Multispectroscopic method. Biomed Environ Sci. 2012 Jun;25(3):334-9

15.   Ji KM, Chen JJ, Gao C, Liu XY, Xia L*, Liu ZG, Li L, Yang SH. A two-site monoclonal antibody immunochromatography assay for rapid detection of peanut allergen Ara h1 in Chinese imported and exported foods. Food chemistry. 2011;129(2):541-5.TOP

16.   Wong JH, Ng TB, Cheung RC, Ye XJ, Wang HX, Lam SK, Lin P, Chan YS, Fang EF, Ngai PH, Xia L*, Ye XY, Jiang Y, Liu F. Proteins with antifungal properties and other medicinal applications from plants and mushrooms. Applied Microbiology and Biotechnology. Appl Microbiol Biotechnol. 2010 Jun 8; ;87(4):1221-35.

17.   Xia L, Willison LN, Porter L, Robotham JM, Teuber SS, Sathe SK, Roux KH. Mapping of a conformational epitope on the cashew allergen Ana o 2: a discontinuous large subunit epitope dependent upon homologous or heterologous small subunit association. Mol Immunol. 2010;47(9):1808-16.

18.   Robotham JM, Xia L, Willison LN, Teuber SS, Sathe SK, Roux KH. Characterization of a cashew allergen, 11S globulin (Ana o 2), conformational epitope. Mol Immunol. 2010;47(9):1830-8.

19.   Liu Z, Xia L, Wu Y, Xia Q, Chen J, Roux KH. Identification and characterization of an arginine kinase as a major allergen from silkworm (Bombyx mori) larvae. Int Arch Allergy Immunol. 2009;150(1):8-14.

20.   Lin P, Wong JH, Xia L, Ng TB. Campesin, a thermostable antifungal peptide with highly potent antipathogenic activities. J Biosci Bioeng. 2009;108(3):259-65.

21.   Wong JH, Xia L, Ng TB. A review of defensins of diverse origins. Curr Protein Pept Sci. 2007;8:446-59.

22.   Lin P, Xia L, Wong JH, Ng TB, Ye X, Wang S, Shi X. Lipid transfer proteins from Brassica campestris and mung bean surpass mung bean chitinase in exploitability. J Pept Sci. 2007;13:642-8.

23.   Lin P, Xia L, Ng TB. First isolation of an antifungal lipid transfer peptide from seeds of a Brassica species. Peptides. 2007;28:1514-9.

24.   Xia L, Ng TB. A hemagglutinin with mitogenic activity from dark red kidney beans. J Chromatogr B Analyt Technol Biomed Life Sci. 2006;844:213-6.

25.   Ng TB, Ngai PH, Xia L. An agglutinin with mitogenic and antiproliferative activities from the mushroom Flammulina velutipes. Mycologia. 2006,98:167-71.

26.   Xia L, Ng TB. An antifungal protein from flageolet beans. Peptides. 2005,26:2397-403.

27.   Xia L, Chu KT, Ng TB. A low-molecular mass ribonuclease from the brown oyster mushroom. J Pept Res. 2005;66:1-8.

28.   Xia L, Ng TB. Isolation of alliumin, a novel protein with antimicrobial and antiproliferative activities from multiple-cloved garlic bulbs. Peptides. 2005;26:177-83.

29.   Chu KT, Xia L, Ng TB. Pleurostrin, an antifungal peptide from the oyster mushroom. Peptides. 2005;26:2098-103.

30.   Xia L, Ng TB. Actinchinin, a novel antifungal protein from the gold kiwi fruit. Peptides. 2004;25:1093-8.

31.   Ng TB, Cheung RC, Ye Xj, Fang EF, Chan YS, Pan WL, Dan XL, Yin CM, Lam SK, Lin P, Ngai PH, Xia L, Liu F, Ye XY, Wang HX, Wong JH.Pharmacotherapy approaches to antifungal prophylaxis. Expert Opin Pharmacother. 2012 Aug;13(12):1695-705. doi:

32.   Ji K, Chen J, Li M, Liu Z, Xia L, Wang C, Zhan Z, Wu X. Comments on serious anaphylaxis caused by nine Chinese herbal injections used to treat common colds and upper respiratory tract infections. Regul Toxicol Pharmacol. 2009;55(2):134-8.

33.   Wu X, Wu H, Xia L, Ji KM, Liu ZG. Socio-technical innovations for total food chain safety during the 2008 Beijing Olympics and Paralympics and beyond. Trends in food science&technology. 2009;21:44-51.

34.   Lin P, Wong JH, Ng TB, Ho VS, Xia L. A sorghum xylanase inhibitor-like protein with highly potent antifungal, antitumor and HIV-1 reverse transcriptase inhibitory activities. Food chemistry. 2013 Dec 1;141(3):2916-22.

35.   Cheung RC, Wong JH, Pan WL, Chan YS, Yin CM, Dan XL, Wang HX, Fang EF, Lam SK, Ngai PH, Xia L, Liu F, Ye XY, Zhang GQ, Liu QH, Sha O, Lin P, Ki C, Bekhit AA, Bekhit Ael-D, Wan DC, Ye XJ, Xia J, Ng TB. Antifungal and antiviral products of marine organisms. Appl Microbiol Biotechnol. 2014 Apr;98(8):3475-94.

36.   Ng TB, Cheung RC, Ye Xj, Fang EF, Chan YS, Pan WL, Dan XL, Yin CM, Lam SK, Lin P, Ngai PH, Xia L, Liu F, Ye XY, Wang HX, Wong JH. Pharmacotherapy approaches to antifungal prophylaxis. Expert Opin Pharmacother. 20