NAME: Kang, Kang

POSITION TITLE: Lecturer; Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Shenzhen University Health Science Center

EDUCATION/TRAINING

A.   Personal Statement

My long-standing research interests are in non-coding RNAs and metabolic diseases, renal disease and pulmonary hypertension .

B.   Research Support

Ongoing Research Support

NSFC (China) project (31571199)                01/01/2016 to 12/31/2019

Kang Kang, PI                                                      ￥630,000 (direct cost)

Role of microRNA in osmoregulation of renal medullary cells via regulating NFAT5 pathway

Completed Research Support

Postdoctoral Science Foundation of China (2013M542203)                  01/01/2013 to 12/31/2014

Kang Kang, PI                                                                                                ￥50,000

Relationship between hypoxia induced downregulation of miR-223 and the development of pulmonary arterial hypertention

Shenzhen Municipal Basic Research Program (JCYJ20130329120507746)                    01/01/2013 to 12/31/2015

Kang Kang, PI                                                             ￥100,000

microRNA Expression pattern in the serum of pulmonary arterial hypertension and noninvasive approach for early diagnosis

C.   Peer-reviewed publications (*: corresponding author)

1. Kang K , Peng X, Zhang X, Wang Y, Zhang L, Gao L, Weng T, Zhang H, Ramchandran R, Raj J U, Gou D* and Liu L. MicroRNA-124 suppresses the transactivation of nuclear factor of activated T cells by targeting multiple genes and inhibits the proliferation of pulmonary artery smooth muscle cells. J Biol Chem 2013, 288(35):25414-25427.c
2. Kang K , Zhang X, Liu H, Wang Z, Zhong J, Huang Z, Peng X, Zeng Y, Wang Y, Yang Y, Luo J and Gou D*. A Novel Real-Time PCR Assay of microRNAs Using S-Poly(T), a Specific Oligo(dT) Reverse Transcription Primer with Excellent Sensitivity and Specificity. PLoS One 2012, 7(11):e48536-48545.
3. Kang K , Zhong J, Jiang L, Liu G, Gou C Y, Wu Q, Wang Y, Luo J, and Gou D*. Identification of microRNA-like RNAs in the Filamentous Fungus Trichoderma reesei by Solexa Sequencing. PLoS One, 2013, 8(10) : e76288-295.
4. Kang K , Peng X, Luo J, Gou D*: Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling. Journal of Animal Science and Biotechnology 2012, 3(4):1-9.
5. Kang K , Wang S, Lai G, Liu G, Xing M*: Characterization of a novel swollenin from Penicillium oxalicum in facilitating enzymatic saccharification of cellulose. BMC Biotechnology 2013, 13(42):1-9.
6. Kang K , Yang K, Zhong J, Tian Y, Zhang L, Zhai J, Zhang L, Song C, Gou CY, Luo J, Gou D*. A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification. J Anim Sci Biotechnol. 2014;5(1):45.
7. Y. Zeng, X. Zhang, K. Kang^{co first}, J. Chen, Z. Wu, J. Huang, W. Lu, Y. Chen, J. Zhang, Z. Wang, Y. Zhai, J. Qu, R. Ramchandran, J.U. Raj, J. Wang, D. Gou*, MicroRNA-223 Attenuates Hypoxia-induced Vascular Remodeling by Targeting RhoB/MLC2 in Pulmonary Arterial Smooth Muscle Cells, Scientific reports, 6 (2016) 24900.
8. Niu Y, Zhang L, Qiu H, Wu Y, Wang Z, Zai Y, Liu L, Qu J, Kang K*and Gou D*: An improved method for detecting circulating microRNAs with S-Poly(T) Plus real-time PCR. Scientific reports 2015, 5:15100.
9. Tang Z, Liu N, Luo L, Zhong J, Kang K, Qiu H, Gou D*. microRNA-17-92 regulates the transcription factor E2F3b during myogenesis in vitro and in vivo. Int J Mol Sci, 2017, 18(4): 1-9.
10. Tang Z, Qiu H, Liu N, Luo L, Zhong J, Kang K, Li L, Gou D*. miR-34b modulates skeletal muscle cell proliferation and differentiation. J Cell Biochem, 2017, 9999: 1-11.
11. Chen, J; Cui X; Qian Z; Li Y, Kang K, Qu J, Li L, Gou D*. Multi-omics analysis reveals regulators of the response to PDGF-BB treatment in pulmonary artery smooth muscle cell. BMC Genomics. 2016; Oct 6;17(1):781.
12. Niu N, Wu Y, Huang J, Li Q, Kang K, Qu J, Li F and Gou D*. Identification of reference genes for circulating microRNA analysis in colorectal cancer. Sci Rep.  2016; Oct 19;6:35611. 
13. Zhang H, Liu, Dong T, Yang J, Wu Y, Xie Y, Kang K, Hu S, Gou D* and Wei Y*. Profiling of differentially expressed microRNAs in arrhythmogenic right ventricular cardiomyopathy. Sci Rep. 2016; Jun 16;6:28101. 
14. Qiu H, Liu N, Luo L, Zhong J, Tang Z, Kang K, Qu J, Peng W, Liu L, Li L and  Gou D*: MicroRNA-17-92 regulates myoblast proliferation and differentiation by targeting the ENH1/Id1 signaling axis. Cell Death and Differentiation .2016 June.
15. Qian Z, Zhang L, Chen J, Li Y, Kang K, Qu J, Wang Z, Zhai Y, Li L, Gou D*. MiR-328 targeting PIM-1 inhibits proliferation and migration of pulmonary arterial smooth muscle cells in PDGFBB signaling pathway. Oncotarget. 2016; June.
16. Qian Z, Wu Z, Huang L, Qiu H, Wang L, Li L, Yao L, Kang K, Qu J, Wu Y, Luo J, Liu JJ, Yang Y, Yang W, Gou D*: Mulberry fruit prevents LPS-induced NF-kappaB/pERK/MAPK signals in macrophages and suppresses acute colitis and colorectal tumorigenesis in mice. Scientific reports 2015, 5:17348.
17. Qiu H, Zhong J, Luo L, Liu N, Kang K, Qu J, Peng W, Gou D*: A PCR-Based Method to Construct Lentiviral Vector Expressing Double Tough Decoy for miRNA Inhibition. PLoS One 2015, 10(12):e0143864.
18. He J, Huang L, Qiu H, Li J, Luo L, Li Y, Tian S, Kang K, Luo J, Liu L, Gou D*. A new design of a lentiviral shRNA vector with inducible co-expression of ARGONAUTE 2 for enhancing gene silencing efficiency. Cell Biosci. 2015 8;5:67.
19. Yan Z, Pan Y, Long H, Kang K, Wu Y, Hui G, Peng W, Gou D*. MiR-20a regulates PRKG1 gene by targeting its coding region in pulmonary arterial smooth muscle cells. FEBS Letters, 2014, 588(24): 4677-4685.
20. Zeng Y, Liu H, Kang K, Wang Z, Hui G, Zhang X, Zhong J, Peng W, Ramchandran R, Usha Raj J, Gou D*: Hypoxia inducible factor-1 mediates expression of miR-322: potential role in proliferation and migration of pulmonary arterial smooth muscle cells. Scientific reports, 2015, 5:12098.
21. Zeng Y, Wang Y, Wu Z, Kang K, Peng X, Peng W, Qu J, Liu L, Raj JU, Gou D*: miR-9 enhances the transactivation of nuclear factor of activated T cells by targeting KPNB1 and DYRK1B. Am J Physiol Cell Physiol, 2015, 308(9):C720-728.
22. Gou D*, Ramchandran R, Peng X, Yao L, Kang K, Sarkar J, Wang Z, Zhou G, Raj J: miR-210 has an anti-apoptotic effect in pulmonary artery smooth muscle cells during hypoxia. Am J Physiol Lung Cell Mol Physiol 2012, 303(8):L682-691.
23. Bao Y, Guo Y, Li Z, Fang W, Yang Y, Li X, Li Z, Xiong B, Chen Z, Wang J, Kang K, Gou D, Yang W*. MicroRNA profiling in Muc2 knockout mice of colitis-associated cancer model reveals epigenetic alterations during chronic colitis malignant transformation. PLoS One, 2014, 9(6):e99132-99142.
24. Liu S, Kang K^{co first}, Zhang J, Ouyang Q, Zhou Z, Tian S, Xing M*: A novel Physarum polycephalum SR protein kinase specifically phosphorylates the RS domain of the human SR protein, ASF/SF2. Acta Biochim Biophys Sin (Shanghai) 2009, 41(8):657-667.
25. Liu S, Li M, Zhang J, Kang K, Tian S, Wang Y, Xing M*: Activation of the transcription of Gal4-regulated genes by Physarum 14-3-3 in yeast is related to dimer-binding motif-2 and three phosphorylation sites. Arch Microbiol 2010, 192(1):33-40.