NAME: Chang, Gang

POSITION TITLE: Assistant Professor of Stem Cell Biology, Shenzhen University School of Medicine

EDUCATION/TRAINING

A.   Personal Statement

My long-standing research interests lie in the mechanisms of reprogramming and regenerative medicine capacity of stem cells. I served as the founding director of the Beijing Key Laboratory of DNA Damage Response from 2011 to 2017.My lab is actively exploring how somatic cells are reprogrammed into pluripotent stem cells and how functional cells are generated from pluripotent stem cells via direct differentiation. Research in my lab has been continuously supported by the National Natural Science Foundation in China (NSFC) and the Science and Technology Planning Project of Guangdong Province. I have published > 10 papers in professional, peer-reviewed journals.

B.   Positions and Honors

Positions and Employment

0 9 / 2009 –1 2 / 2011        Assistant Professor, Capital Medical School, Beijing, China

0 1 /201 2 – 0 6 /201 3      Senior Investigator, National Institute of Biological Sciences, Beijing (NIBS), Beijing, China

0 7 /201 3 – present       Assistant Professor, Shenzhen University School of Medicine, Shenzhen, China

Other Experience and Honors

1.    Outstanding Graduates of Chinese Academic Sciences: 2009

2.    Reserve Talent of High-level Professionals in Shenzhen: 2014-present

C.   Contributions to Science (*: corresponding/co-corresponding author)

A full list of my publications (in a total of 12) is enclosed at the end of this document.

1.    Nuclear reprogamming via nuclear transfer and transcription factors

1)    Chang G, Liu S, Wang F, Zhang Y, Kou Z, Chen D*, Gao S*. 2009. Differential methylation status of imprinted genes in nuclear transfer derived ES (NT-ES) cells. Genomics 93, 112-119.

2)    Ding J, Guo Y, Liu S, Yan Y, Chang G, Kou Z, Zhang Y, Jiang Y, He F, Gao S, Sang J. 2009. Embryonic stem cells derived from somatic cloned and fertilized blastocysts are post-transcriptionally indistinguishable: a MicroRNA and protein profile comparison. Proteomics 9(10):2711-2721.

3)    Chang G＃, Miao YL＃, Zhang Y, Liu S, Kou Z, Ding J, Chen DY, Sun QY*, Gao S*. 2010. Linking incomplete reprogramming to the improved pluripotency of murine embryonal carcinoma cell-derived pluripotent stem cells. PloS One 5, e10320.

4)    Gao S＃, Wang ZL＃, Di KQ＃, Chang G, Tao L, An L, Wu FJ, Xu JQ, Liu YW, Wu ZH, Li XY, Gao S*, Tian JH*. 2013. Melatonin improves the reprogramming efficiency of murine-induced pluripotent stem cells using a secondary inducible system. Journal of Pineal Research 55, 31-39.  

5)    Tao Y＃, Zheng W＃, Jiang Y＃, Ding G＃, Hou X, Tang Y, Li Y, Gao S, Chang G, Zhang X, Liu W, Kou X, Wang H, Jiang C*, Gao S*. 2014. Nucleosome organizations in induced pluripotent stem cells reprogrammed from somatic cells belonging to three different germ layers. BMC Biol 12:109.

6)    Di KQ＃, Gao S＃, Cui LF＃, Chang G, Wu FJ, Ren LK, An L, Miao K, Tan K, Tao L, Chen H, Wang ZL, Wang SM, Wu ZH, Gao S, Li XY*, Tian JH*. 2015. Generation of fully pluripotent female murine-induced pluripotent stem cells. Biol Reprod 92:123.

2.    Epigenetic dynamics in reprogramming

1)    Chang G＃, Gao S＃, Hou X, Xu Z, Liu Y, Kang L, Tao Y, Liu W, Huang B, Kou X, Chen J, An L, Miao K, Di K, Wang Z, Tan K, Cheng T, Cai T, Gao S*, Tian J*. 2014. High-throughput sequencing reveals the disruption of methylation of imprinted gene in induced pluripotent stem cells. Cell Research 24, 293-306.

2)    Gao S＃, Zheng C＃, Chang G＃, Liu W, Kou X, Tan K, Tao L, Xu K, Wang H, Cai J, Tian J*, Gao S*. 2015. Unique features of mutations revealed by sequentially reprogrammed induced pluripotent stem cells. Nature Communications 6, 6318.

3.    Reprogramming of HSC/HPCs

1)    Gao S, Tao L, Hou X, Xu Z, Liu W, Zhao K, Guo M, Wang H, Cai T, Tian J, Gao S, Chang G*. 2016. Genome-wide gene expression analyses reveal unique cellular characteristics related to the amenability of HPC/HSCs into high-quality induced pluripotent stem cells. Stem Cell Res Ther. 7:40.

2)    Gao S＃, Hou X＃, Jiang Y, Xu Z, Cai T, Chen J*, Chang G*. 2017. Integrated analysis of hematopoietic differentiation outcomes and molecular characterization reveals unbiased differentiation capacity and minor transcriptional memory in HPC/HSC-iPSCs. Stem Cell Res Ther. 8:13.

4.    A nti-cancer research

1)    Cai N, Zhou W, Ye LL, Chen J, Liang QN, Chang G*, Chen JJ* (2017). The STAT3 inhibitor pimozide impedes cell proliferation and induces ROS generation in human osteosarcoma by suppressing catalase expression. Am J Transl Res. 9(8):3853-3866.

2)    Chen JJ*, Zhou W, Cai N, Chang G* (2017). In Vivo Murine Model of Leukemia Cell-Induced Spinal Bone Destruction. Biomed Res Int. 2017:3521481.

D.   Research Support

Ongoing Research Support

Science and Technology Planning Project of Guangdong Province (2016A020214020) 

01/06/2016 to 05/31/2018

Gang Chang, PI                                                          ￥300,000

Genetic mutation during the sequential reprogramming of somatic cells

Medical Scientific Research Foundation of Guangdong Province (A2017096)                

01/07/2017 to 06/31/2019

Gang Chang, PI                                                           ￥10,000

Establishment of the quality control system of high-quality iPSCs

Completed Research Support

NSFC (China) (31000656)                                          01/01/2011 to 12/31/2013

Gang Chang, PI                                                           ￥180,000

The derivation of iPSCs from adipose stem cells

E.    Peer-reviewed publications ( ＃ : first author *: corresponding author)

1.      Chen JJ*, Zhou W, Cai N, Chang G* (2017). In Vivo Murine Model of Leukemia Cell-Induced Spinal Bone Destruction. Biomed Res Int. 2017:3521481.

2.      Cai N, Zhou W, Ye LL, Chen J, Liang QN, Chang G*, Chen JJ* (2017). The STAT3 inhibitor pimozide impedes cell proliferation and induces ROS generation in human osteosarcoma by suppressing catalase expression. Am J Transl Res. 9(8):3853-3866.

3.      Gao S＃, Hou X＃, Jiang Y, Xu Z, Cai T, Chen J*, Chang G*. 2017. Integrated analysis of hematopoietic differentiation outcomes and molecular characterization reveals unbiased differentiation capacity and minor transcriptional memory in HPC/HSC-iPSCs. Stem Cell Res Ther. 8:13.

4.      Gao S, Tao L, Hou X, Xu Z, Liu W, Zhao K, Guo M, Wang H, Cai T, Tian J, Gao S, Chang G*. 2016. Genome-wide gene expression analyses reveal unique cellular characteristics related to the amenability of HPC/HSCs into high-quality induced pluripotent stem cells. Stem Cell Res Ther. 7:40.

5.      Gao S＃, Zheng C＃, Chang G＃, Liu W, Kou X, Tan K, Tao L, Xu K, Wang H, Cai J, Tian J*, Gao S*. 2015. Unique features of mutations revealed by sequentially reprogrammed induced pluripotent stem cells. Nature Communications 6, 6318.

6.      Di KQ＃, Gao S＃, Cui LF＃, Chang G, Wu FJ, Ren LK, An L, Miao K, Tan K, Tao L, Chen H, Wang ZL, Wang SM, Wu ZH, Gao S, Li XY*, Tian JH*. 2015. Generation of fully pluripotent female murine-induced pluripotent stem cells. Biol Reprod 92:123.

7.      Chang G＃, Gao S＃, Hou X, Xu Z, Liu Y, Kang L, Tao Y, Liu W, Huang B, Kou X, Chen J, An L, Miao K, Di K, Wang Z, Tan K, Cheng T, Cai T, Gao S*, Tian J*. 2014. High-throughput sequencing reveals the disruption of methylation of imprinted gene in induced pluripotent stem cells. Cell Research 24, 293-306.

8.      Tao Y＃, Zheng W＃, Jiang Y＃, Ding G＃, Hou X, Tang Y, Li Y, Gao S, Chang G, Zhang X, Liu W, Kou X, Wang H, Jiang C*, Gao S*. 2014. Nucleosome organizations in induced pluripotent stem cells reprogrammed from somatic cells belonging to three different germ layers. BMC Biol 12:109.

9.      Gao S＃, Wang ZL＃, Di KQ＃, Chang G, Tao L, An L, Wu FJ, Xu JQ, Liu YW, Wu ZH, Li XY, Gao S*, Tian JH*. 2013. Melatonin improves the reprogramming efficiency of murine-induced pluripotent stem cells using a secondary inducible system. Journal of Pineal Research 55, 31-39.  

10.   Chang G＃, Miao YL＃, Zhang Y, Liu S, Kou Z, Ding J, Chen DY, Sun QY*, Gao S*. 2010. Linking incomplete reprogramming to the improved pluripotency of murine embryonal carcinoma cell-derived pluripotent stem cells. PloS One 5, e10320.

11.   Ding J, Guo Y, Liu S, Yan Y, Chang G, Kou Z, Zhang Y, Jiang Y, He F, Gao S, Sang J. 2009. Embryonic stem cells derived from somatic cloned and fertilized blastocysts are post-transcriptionally indistinguishable: a MicroRNA and protein profile comparison. Proteomics 9(10):2711-2721.

12.   Chang G, Liu S, Wang F, Zhang Y, Kou Z, Chen D*, Gao S*. 2009. Differential methylation status of imprinted genes in nuclear transfer derived ES (NT-ES) cells. Genomics 93, 112-119.