NAME: Ji, Kunmei

POSITION TITLE: Professor

EDUCATION/TRAINING

A.   Personal Statement

I received my bachelor’s degree in 1989 from Nanjing University and my Master’s degree in 1991 from Sun Yat-sen University. In 1999, I received my doctorate from Sun Yat-Sen University. I served as the Director of the Center for Biomedical Engineering of 999 Group from 1997 to 2000. In October 2007, I joined the Medical school of Shenzhen University and served as the Director of the Department of Biochemistry and Molecular Biology. In 2010, I was awarded the qualification of Basic Medical Professor by the Personnel Office of the Guangdong province government. Since 2011, I have been employed by Shenzhen University as a Professor of Basic Medicine.

I have been engaged in the basic research of allergic reaction and the application of translational immunology. I have received several achievements in this area and in vitro diagnostic reagent development and application. I have published 23 SCI papers and 1 SSCI paper in journals including NEJM, JACI, and Allergy, of which 8 papers are JCR class one. One of the articles was selected for the Hong Kong medical ee-education study paper, and two were cited by the World Allergy Organization's Guideline for 2011. I identified the allergens Derf24 and Derp24 for the first time in and analyzed the dust mite genome and transcriptome. My published research on this topic were selected as the Editor’s choice and a related editorial was published highlighting my work: "Studies of house dust mites can now fully embrace the ‘‘-omics’’ era" (JACI, 2015, Vol 135 (2) : 369,549-550). In addition, this research was selected by the National Natural Science Foundation of China in the journal Science Foundation in China (2015, Vol.23(4) : 12).

I have received eight grants to fund my research: 1 NSFC project, 4 Guangdong Province science and Technology projects and 3 Shenzhen Science and Technology projects. I won first prize of the Guangdong Province Science and Technology Progress Award, and second prize of Science and Technology Progress Award of the Chinese Ministry of Education. I also opened a web lecture of biological chemistry and molecular biology (WeChat: BioChem_SZU). My research group covers a comprehensive range of techniques, including protein crystallization, genome analysis, diagnostics and vaccine development, and cultivates high-level postgraduates.

B.   Positions and Honors

Positions and Employment

08/1995 – 10/2001      Director of the Center, Biomedical Engineering of 999 Group, Shenzhen, China

02/2001 – 05/2003      Technical Director, Yuan Dongli company, Shenzhen, China

06/2003 – 09/2007      Researcher, Anaphylaxis Institute of Shenzhen University , Shenzhen, China

10/2007 – present      Director of the Department of Biochemistry and Molecular Biology, Medical school of Shenzhen University, Shenzhen, China

C.   Contributions to Science (*: corresponding/co-corresponding author)

[1] Pengfei Wei, Zelang Cai, Jinwen Hua, Weijia Yu, Jiajie Chen, Kang Kang, Congling Qiu, Lanlan Ye, Jiayun Hu, Kunmei Ji*.Pains and Gains from China’s Experiences with Emerging Epidemics: From SARS to H7N9. BioMed Research International.Volume 2016 (2016), Article ID 5717108, 6 pages cited 0

http://dx.doi.org/10.1155/2016/5717108

[2] Ma YJ, Zhang HD, Wu CH, Zhu GL, Ji YQ, Huang JL, Du LT, Cao P, Zang DY, Ji KM.Rapid CO breath test screening of drugs for protective effects on ribavirin-induced hemolysis in a rabbit model: a pilot study. J Breath Res. 2016 Aug 10;10(3):036010. doi: 10.1088/1752-7155/10/3/036010. cited 0

[3]Chan TF ， Ji KM （ co-first ） , Yim AK ， Liu XY ， Zhou JW, Li RQ, Yang KY, Li J, Li M, Law PT, Wu YL, Cai ZL, Qin H, Bao Y, Leung RK, Ng PK, Zou J, Zhong XJ, Ran PX, Zhong NS, Liu ZG, Tsui SK. The draft genome, transcriptome, and microbiome of Dermatophagoidesfarinae reveal a broad spectrum of dust mite allergens. J Allergy ClinImmunol. 2015 Feb;135(2):539-48.

[4] Xuli W, Weiyi H, Ji KM, Wenpu W, Dongsheng H, Hui W, Xinpin L, Zhigang L ， A simple and fast detection method for bovine milk residues in foods: a 2-site monoclonal antibody immunochromatography assay.J Food Sci.78(3):M452-7. 2013 （ IF1.791 ） cited 0

[5] JiaJie Chen, Lixin Xia, Xuli Wu, Suhan Yang,Ji KM*, ZhiGang Liu, MuJin Tang, Chen Gao ， A practical test system for sensitive, rapid screening and authentication of peanut allergens in imported and exported food products in Chinese Customs. Food Control, 23 （ 1 ） , pp154-158, 2012 （ IF 3.388 ） cited 0

[6] Ji KM(co-first), Jia-Jie Chen, Chen Gao, Xiao-yu Liu, Li-xin Xia,  Zhi-Gang Liu,  Liang Li and Suhan Yang. A two-site monoclonal antibody immunochromatography assay for rapid detection of peanut allergen Ara h1 in Chinese imported and exported foods. Food Chemistry, 129, pp541-545, 2011

[7] Mao Y, Lin J, Zhou A, Ji KM, Downey JS, Chen R, Han A.Quikgene: A gene synthesis method integrated with ligation-free cloning. Anal Biochem, 415 （ 1 ） , pp21-26, 2011 （ IF2.305 ） cited 7

[8] Zhan ZK, Ji KM （ co-first ） , Liu XY, Liu ZG, Li M, Chen JJ, Li JN, Qiu S. Monoclonal antibodies against recombinant Der f 3 reveal localization of Der f 3 in the gut and faecal pellets of Dermatophagoides farinae. Experimental and Applied Acarology, 52(1), pp63-71, 2010

[9]Dai Bin; Ji Kun-Mei; Zhang Tong-Yan;Peng Xiao-Jun . Optimized Conditions of Protein Prestained with Reactive Dye in Native Polyacrylamide Gel Electrophoresis. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,38(10) ,pp1423-1427, 2010

[10] Wu X, Wu H, Xia L,Ji KM*, Liu ZG*,Chen JJ, Hu DS, Gao C, Wu Y. Socio-technical innovations for total food chain safety during the 2008 Beijing Olympics and Paralympics and beyond. Trends in food science&technology, 21, pp44-51, 2009

[11] Ji KM Li M, Chen JJ, Zhan ZK, Liu ZG. Anaphylactic shock and lethal anaphylaxis caused by Houttuynia Cordata injection, a herbal treatment in China. Allergy, 64(5), pp816-7, 2009 （ IF5.995 ）

[12] Ji KM, Jiajie Chen, Meng Li, Zhigang Liu, Chunbo Wang, Zhengke Zhan, Xuli Wu and Qingyou Xi. Anaphylactic shock and lethal anaphylaxis caused by food consumption in China.Trends in Food Science & Technology, 20(5), pp227-231, 2009

[13]Ji KM, Chen J, Li M, Liu Z, Xia L, Wang C, Zhan Z, Wu X. Comments on serious anaphylaxis caused by nine Chinese herbal injections used to treat common colds and upper respiratory tract infections. RegulToxicolPharmacol, 55(2), pp134-8, 2009

[14] Dey R, Ji KM, Liu Z, Chen L. A cytokine-cytokine interaction in the assembly of higher-order structure and activation of the interleukine-3:receptor complex. PLoS One, 4 （ 4 ） , pp5188, 2009

[15] Meng Li, Ji KM, Zhigang Liu, Chunbo Wang, Jiajie Chen, Zhengke Zhan and Xuli Wu.Food allergen labeling during the 2008 Beijing Olympics and Paralympics and beyond. Trends in Food Science & Technology, 20(2), pp100-102, 2009

[16] Yu HQ, Li XH, Guo H, Liu ZG, Ran PX, Ji KM, Wang J.Sublingual Immunotherapy Efficacy of Dermatophagoidesfarinae Vaccine in a Murine Asthma Model. Int Arch Allergy Immunol, 152(1), pp41-48, 2009

[17] Ji KM, Zhan ZK, Chen JJ, Liu ZG. Anaphylactic shock caused by silkworm pupa consumption in China. Allergy, 63(10), pp1407-8, 2008

[18] Liu Z, Bai Y, Ji KM, Liu X, Cai C, Yu H, Li M, Bao Y, Lian Y, Gao B, Detection of Dermatophagoides Farinae in the Dust of Air-conditioning filters. International Archives of Allergy and Immunology, 144, pp85-90, 2007

[19] Shi S, Lin J, Cai Y, Yu J, Hong H, Ji KM, Downey JS, Lu X, Chen R, Han J, Han A. Dimeric structure of p300/CBP associated factor.BMCStruct Biol. 2014 Jan 14;14:2. （ IF2.222 ） cited 7

[20] Du JH, Zhang HD, Ma ZJ, Ji KM. Artesunate induces oncosis-like cell death in vitro and has antitumor activity against pancreatic cancer xenografts in vivo.Cancer chemotherapy and Pharmacology,65(5):895-902,2010

[21] Ji KM , Zhu S, Liu Z. Melamine-Contaminated Powdered Formula and Urolithiasis.Correspondence. N Engl J Med, 360, pp2675-2676, 2009 （ Correspondence ）

[22] Zhu SL, Li JH, Chen L, Bao ZX, Zhang LJ, Li JP, Chen JH, Ji KM*. Conservative Management of Pediatric Nephrolithiasis Caused by  Melamine-Contaminated Milk Powder. Pediatrics, 123, pp1099-1102, 2009

[23] Ji KM*, Li CL, Meng G, Han AD, Wu XL. New BZLF1 sequence variations in EBV-associated undifferentiated nasopharyngeal carcinoma in southern China. Arch Virol, 153(10), pp1949-53, 2008

[24] Junfang Zhang, Zelang Cai, Zhenfu Zhao , Kunmei Ji*. Cell phone-based online biochemistry and molecular biology medical education curriculum . 2017, 22 (1). Article: 1374135, doi.org/10.1080/10872981.2017.1374135. Published online: 13 Sep 2017.

D.   Research Support

Ongoing Research Support

NSFC (China) general program (81571570)               01/01/2016 to 12/31/2019

Kunmei Ji, PI                                                               ￥600,000 (direct cost)

High Throughput Analysis of IgE Reactive Protein Group of Dust Mites and New Derf24 Sensitization Mechanism

Science and technology plan project public welfare fund and ability construction project of Guangdong (2017A010105014)                   01/01/2017 to 12/31/2018

Kunmei Ji, PI                                                               ￥300,000 (direct cost)

Cardiovascular and Cerebrovascular Events Early Warning and Diagnosis Reagent Lp-PLA2 Industrialization

Guangdong Natural Science Foundation Free Application Project (2016A030313039)                                                                                                06/01/2016 to 06/30/2019

Kunmei Ji , PI                                                               ￥100,000

Molecular Mechanism of Derf24 Sensitization of Newly Dust Mite Allergen

Guangdong Natural Science Foundation Free Application Project (2014566)                                                                                                             01/01/2015 to 12/31/2017

Kunmei Ji , PI                                                               ￥100,000

Identification of New Derf23 Allergenicity and Preliminary Mapping of Dust Mite Allergens

Shenzhen Basic Research Project (JCYJ20150626141652681)           01/01/2016 to 12/31/2019

Kunmei Ji, PI                                                                 ￥300,000

Derf24 Promotes the Molecular Mechanism of TSLP Sensitization via the STIM1/ORAI1/NFAT Pathway

Beijing Municipal Support for Creative Teams    

Completed Research Support

Guangdong High-tech Industrialization Project (2009B011300010)              01/01/2010 to 12/31/2011

Kunmei Ji, PI                                                                        ￥50,000

Development of a rapid quantitative anti-counterfeiting detection kit for milk-specific proteins

Shenzhen Basic Research Project (JCYJ20130326112225593)          10/01/2013 to 09/30/2015

Kunmei Ji, PI                                                                        ￥100,000

Identification of New Derf23 Allergenicity and Preliminary Mapping of Dust Mite Allergens

Shenzhen Basic Research Project (JCYJ20120613113021045)           10/01/2012 to 09/30/2014

Kunmei Ji, PI                                                                        ￥100,000

Molecular Mechanism of the Interaction between TSLP and Its Receptors to Study Anti-allergic Inflammatory Drug Targets