NAME: Peng, Yin

POSITION TITLE: Assistant Professor of Pathology; Shenzhen University School of Medicine

EDUCATION/TRAINING

A.   Personal Statement

My long-standing research interests lie in the molecular mechanisms of cell signaling transduction and tumorigenesis. My lab is actively exploring how oncogenic microRNAs and oncogenic circRNAs regulate critical signaling transduction pathways, such as the Wnt/b-catenin pathway and NF-kB signaling pathway to promote cell survival, proliferation and migration in gastric cancer. Research in my lab has been supported by the National Natural Science Foundation in China and Gangdong province  Science Foundation. I have published eight first author/corresponding author papers in professional, peer-reviewed journals.

B.   Positions and Honors

Positions and Employment

08/2011–10/2012        Research fellow, BGI-shenzhen

10/2012 – 11/2014      Research fellow, Shenzhen second people’s hospital

12/2014 – 04/2017      Postdoctoral fellow, Shenzhen Univeristy/Wuhan Univeristy

05/2017–present       Assistant Professor, Shenzhen University, Shenzhen, China

C.   Contributions to Science (*: corresponding/co-corresponding author)

1. Notch-1 role in cervical cancer

Notch-1 inhibits apoptosis in some transformed cells via mechanisms that are incompletely understood. Notch-1 can increase nuclear factor-kappa B (NF-kB) activity through a variety of mechanisms. Over-expression of cleaved Notch-1 in T-cell acute lymphoblastic leukemia cells activates NF-kB via interaction with the I kappa B kinase (IKK) signalosome. Concomitant activation of the Notch and NF-kB pathways has been described in a large series of cervical cancer specimens. Our lab has shown that wild-type, spontaneously expressed Notch-1 stimulates NF-kB activity in CaSki cervical cancer cells by associating with the IKK signalosome through IKKa. A significant fraction of tumor necrosis factor (TNF)-alpha-stimulated IkappaB kinase activity in CaSki cells is Notch-1-dependent. In addition, Notch-1 is found in the nucleus in association with IKKa at IKKa-stimulated promoters and is required for association of IKKa with these promoters under basal and TNF-a-stimulated conditions. Notch-1–IKKa complexes are found in normal human keratinocytes as well, suggesting that IKK regulation is a physiological function of Notch-1. Both Notch-1 and IKKa knockdown sensitize CaSki cells to cisplatin-induced apoptosis to equivalent extents. Our data indicate that Notch-1 regulates NF-kB in cervical cancer cells at least in part via cytoplasmic and nuclear IKK-mediated pathways.

Song, L L#，Peng, Y#，Yun, J，Rizzo, P，Chaturvedi, V，Weijzen, S，Kast, W M，Stone, P J B，Santos, L，Loredo, A，Lendahl, U，Sonenshein, G，Osborne, B，Qin, J-Z，Pannuti, A，Nickoloff, B J，Miele, L*，Notch-1 associates with IKKalpha and regulates IKK activity in cervical cancer cells，Oncogene，2008，27（44）：5833-5844

2. MircoRNAs’ function in gastric cancer

Gastric cancer (GC) is one of the leading causes of cancer-related deaths throughout China and worldwide. The discovery of microRNAs (miRNAs) has provided a new opportunity for developing diagnostic biomarkers and effective therapeutic targets in GC. By performing microarray analyses of benign and malignant gastric epithelial cell lines, we found 16 significantly dysregulated miRNAs; 11 of these were validated by real-time qRT-PCR. Based on miRWalk online database scans, 703 potential mRNA targets of the16 miRNAs were identified. Bioinformatic analyses suggested that these dysregulated miRNAs and their predicted targets were principally involved in tumor pathogenesis, MAPK signaling, and apoptosis( Oncotarget 2015). Among the identified miRNAs, miRNA-194 was over-expressed in GC cell lines and 43 paired GC tissues. We found that miRNA-194 is oncogenic and promotes GC cell proliferation and migration by activating Wnt signaling, at least in part, via suppression of SUFU (Cancer letters 2017, Oncotarget 2016). We also found that SMG-1 is suppressed by miR-192 and-215 and functions as a tumor suppressor in GC by inactivating Wnt signaling and suppressing EMT(Cancer medicine 2018).

(1) Peng,Y^#，Zhang,X^#，Ma,Q^#，Yan,R，Qin,Y，Zhao,Y，Cheng,Y，Yang,M，Wang,Q，Feng,X，Huang,Y，Huang,W，Zhao,Z，Wang,L，Wei,Y，He,Z，Fan,X，Li,S，Jin,Z^*，S.J,Meltzer，MicroRNA-194 activates the Wnt/beta-catenin signaling pathway in gastric cancer by targeting the negative Wnt regulator, SUFU, Cancer letters，385 (2017) 117-127

(2) Peng,Y^#， Zhang,X^#， Feng,X，Fan,X，Jin,Z^*，The crosstalk between microRNAs and the Wnt/beta-catenin signaling pathway in cancer，Oncotarget，2016，8(8):14089-14106

(3) Zhang X#, Peng Y#, Huang Y, Yang M, Yan R, Zhao Y, Cheng Y, Liu X, Deng S, Feng X, Lin H, Yu H, Chen S, Zhao Z, Li S, Li K, Wang L, Wei Y, He Z, Fan X, Meltzer SJ, Li S, Jin Z, SMG-1 inhibition by miR-192/-215 causes epithelial-mesenchymal transition in gastric carcinogenesis via activation of Wnt signaling. Cancer Med. 2017 Dec 13.

(4) Zhang X^#，Peng Y^#，Jin Z^#*，Huang W，Cheng Y，Liu Y，Feng X，Yang M，Huang Y，Zhao Z，Wang L，Wei Y，Fan X，Zheng D^*，Meltzer SJ，Integrated microRNA profiling and bioinformatics analyses reveal potential causative microRNAs in gastric adenocarcinoma，Oncotarget，2015，6(32): 32878- 3289

D.   Research Support

Ongoing Research Support

NSFC (China) (31601028)                                   01/01/2017 to 12/31/2020

Yin Peng, PI                                                                ￥190,000 (direct cost)

The mechanism by which hsa_circ_001634/miR-194/Wnt axis regulates gastric cancer oncogenesis and progression

Guangdong province Science Foundation (2017A030313479)                     05/01/2017 to 05/01/2020

Yin Peng, PI                                                                                                                 ￥100,000 (direct cost)

The molecular mechanism by which RNA hsa_circ_001634 regulates gastric cancer oncogenesis and progression by sponging miR-194

Completed Research Support

China postdoctoral science foundation (2015M582417)           11/2015-11/2016

Yin Peng, PI                                                                                            ￥50,000 (direct cost)

microRNA-194 regulates Wnt signaling pathway in gastric cancer

Schmitt Fellowship                                                08/2008-08/2009

Yin Peng, PI                                                                $18,000 (direct cost)

Notch-1 activates NF-κB in cervical cancer

E.    Peer-reviewed publications (*: corresponding author)

(1)Peng,Y^#，Zhang,X^#，Ma,Q^#，Yan,R，Qin,Y，Zhao,Y，Cheng,Y，Yang,M，Wang,Q，Feng,X，Huang,Y，Huang,W，Zhao,Z，Wang,L，Wei,Y，He,Z，Fan,X，Li,S，Jin,Z^*，S.J,Meltzer，MicroRNA-194 activates the Wnt/beta-catenin signaling pathway in gastric cancer by targeting the negative Wnt regulator, SUFU, Cancer letters，385 (2017) 117-127

(2)Peng,Y^#， Zhang,X^#， Feng,X，Fan,X，Jin,Z^*，The crosstalk between microRNAs and the Wnt/beta-catenin signaling pathway in cancer，Oncotarget，2016，8(8):14089-14106

(3) Zhang X#, Peng Y#, Huang Y, Yang M, Yan R, Zhao Y, Cheng Y, Liu X, Deng S, Feng X, Lin H, Yu H, Chen S, Zhao Z, Li S, Li K, Wang L, Wei Y, He Z, Fan X, Meltzer SJ, Li S, Jin Z, SMG-1 inhibition by miR-192/-215 causes epithelial-mesenchymal transition in gastric carcinogenesis via activation of Wnt signaling. Cancer Med. 2017 Dec 13.

(4) Zhang X^#，Peng Y^#，Jin Z^#*，Huang W，Cheng Y，Liu Y，Feng X，Yang M，Huang Y，Zhao Z，Wang L，Wei Y，Fan X，Zheng D^*，Meltzer SJ，Integrated microRNA profiling and bioinformatics analyses reveal potential causative microRNAs in gastric adenocarcinoma，Oncotarget，2015，6(32): 32878- 3289

(5) Song, L L^#，Peng, Y^#，Yun, J，Rizzo, P，Chaturvedi, V，Weijzen, S，Kast, W M，Stone, P J B，Santos, L，Loredo, A，Lendahl, U，Sonenshein, G，Osborne, B，Qin, J-Z，Pannuti, A，Nickoloff, B J，Miele, L^*，Notch-1 associates with IKKalpha and regulates IKK activity in cervical cancer cells，Oncogene，2008，27（44）：5833-5844

(6) Guan, Y#，Hu, H#，Peng, Y#，Gong, Y，Yi, Y，Shao L，Liu, T，Li, G，Wang, R，Dai, P，Bignon, Y，Xiao, Z，Yang, L，Mu, F，Xiao, L，Xie, Z，Yan, W，Xu, N，Zhou, D*，Yi, X*，Detection of inherited mutations for hereditary cancer using target enrichment and next generation sequencing，Fam Cancer，2014，106（014）

(7)Wang C, Xiao L, Han J, Jin CE, Peng Y and Yang Z. A prospective randomized trial of selective versus nonselective esophagogastric devascularization for portal hypertension. Journal of Huazhong University of Science and Technology Medical sciences 2014; 34(4):563-568.

(8)Guan YF,Li GR,Wang RJ, Yi YT,Yang L, Jiang D, Zhang XP and Peng Y*. Application of next-generation sequencing in clinical oncology. Chinese Journal of Cancer. 2012; 31(10):463-470.

(9)Wang L#, Zhang X#, Peng Y#, Gao Y, Zhang Y, Feng X, Chen S, Yu H, Huang W, Huang Y, Jian Q, Zhao Z, Fan X and Jin Z. Aberrant Methylation of HLTF Gene in Human Esophageal Cancer. Int J Hum Genet. 2016;16(1,2):70-76.