NAME: Liu, Lizhong

POSITION TITLE: Associate Professor of Physiology; Director of XiLi Testing Center, ShenZhen University

EDUCATION/TRAINING

INSTITUTION AND LOCATION	DEGREE (if applicable)	Completion Date MM/YYYY	FIELD OF STUDY
Jilin University, Jilin, China	B.Sc.	07/1994	Biochemistry
The Academy of Military Medical Sciences of Chinese PLA , China	M.Sc.	07/1997	Biochemistry and Molecular Biology
Chinese Academy of Medical Science & Peking Union Medical College, China	Ph.D.	07/2005	Biochemistry and Molecular Biology
The Chinese University of Hong Kong, Hong Kong University Liberty of Brussels, Brussels	Postdoctoral Postdoctoral	02/2011 12/2011	Diabetes Diabetes

A.   Personal Statement

I was appointed as Associate Professor and Master Tutor by Shenzhen University in 2013. In the same year, I received the Shenzhen Overseas High-level Talent Award. I am currently serving as the Director of the XiLi Testing Center and Deputy Director of the Teaching and Research Center, Shenzhen University. My research mainly focuses on the pathogenesis of diabetes, diabetes drug development and tumor metabolism. My research team has made independent discoveries and achievements in insulin signaling transduction, apoptosis of islet beta cells, the mechanisms of anti-diabetic drugs and tumor metabolism. Combined, my team has vast professional knowledge and experimental expertise with regards to studying dynamic changes of the cytoskeleton and vesicle transportation of glucose and fatty acids. I use modern cellular and molecular technologies to assess and select anti-diabetic and anti-cancer drugs both in vitro and in vivo, and new drugs have been discovered based on my pharmacological research. My projects are supported by the National Natural Science Foundation of China (NSFC), the Overseas High-level Talent Foundation and the Basic Research Foundation of Shenzhen. I have published my research in high impact SCI journals, such as Mol. Cell. Endocrinol, Cell Signal and Mol. Biol. Cell, and  I am currently mentoring three MSc candidates.

 

B.   Positions and Honors

Positions and Employment

08/1997 – 07/2002      Attending Doctor, General Airforce Hospital, Beijing, China

      12/2004 – 08/2005  Visiting Scientist, The Chinese University of Hong Kong, Hong Kong

01/2012 – 03/2012      Research Associate, The Chinese University of Hong Kong, Hong Kong

04/2012 – present      Associate Professor, School of Medicine, Shenzhen University, Shenzhen, China

05/2015 – present              Deputy Director of the Teaching and Research Center, School of Medicine,

                                   Shenzhen University, Shenzhen, China

05/2016 – present       Director of the XiLi Testing Center, Shenzhen University, Shenzhen, China

 

C.   Research Support

Ongoing Research Support

NSFC (China) general project (81570784)           01/01/2016 to 12/31/2019

Lizhong, Liu  PI                                                               ￥696,000 (direct cost)

Functional study of PKCzeta in regulating high fat-induced CD36 translocation and its relationship with insulin resistance in skeletal muscle cells

 

Completed Research Support

      Shenzhen Oversea High-level Talent Fund (8270000003)       01/12/2013 to 12/31/2015

Lizhong, Liu, PI                                                                                ￥3000,000

The distribution of GLUT4 and CD36 on homeostasis of glucose and lipid metabolism

 

Shenzhen Basic Research Fund (JCYJ20150324141711688)   07/01/2004 to 06/30/2006

Lizhong, Liu, PI                                                                                 ￥300,000

Mechanism study of abnormal distribution of CD36 during high fat induced insulin resistance

 

D.   Peer-reviewed publications

        Feng Y, Lv M, Lu Y, Liu K, Liu LZ, He Z, Wu K, Wang X, Zhang B, Wu X. Characterization of binding interactions between selected phenylpropanoid glycosides and trypsin. Food Chem. 2018 Mar; 243:118-124.

 

Zheng K, Jiang Y, Liao C, Hu X, Li Y, Zeng Y, Zhang J, Wu X, Wu H, Liu LZ, Wang Y, He Z. NOX2-mediated TFEB activation and vacuolization regulate lysosome-associated cell death induced by Gypenoside L, a saponin isolated from Gynostemma pentaphyllum. J Agric Food Chem. 2017 Aug; 65(31):6625-6637

 

Lu Y, Zhou W, Feng Y, Li Y, Liu K, Liu LZ, Lin D, He Z, Wu X. Acteoside and Acyl-Migrated Acteoside, Compounds in Chinese Kudingcha Tea, Inhibit α-Amylase In Vitro. J Med Food. 2017 Jun;20(6):577-585.

 

Yang S, Liu Y, Li MY, Ng CSH, Yang SL, Wang S, Zou C, Dong Y, Du J, Long X, Liu LZ, Wan IYP, Mok T, Underwood MJ, Chen GG. FOXP3 promotes tumor growth and metastasis by activating Wnt/β-catenin signaling pathway and EMT in non-small cell lung cancer. Mol Cancer. 2017 Jul;16(1):124-136

 

Song X, He J, Xu H, Hu XP, Wu XL, Wu HQ, Liu LZ, Liao CH, Zeng Y, Li Y, Hao Y, Xu CS, Fan L, Zhang J, Zhang HJ, He ZD. The antiviral effects of acteoside and the underlying IFN-γ-inducing action. Food Funct. 2016 Jul; 7(7):3017-30

 

Liao C, Zheng K, Li Y, Xu H, Kang Q, Fan L, Hu X, Jin Z, Zeng Y, Kong X, Zhang J, Wu X, Wu H, Liu LZ, Xiao X, Wang Y, He Z. Gypenoside L inhibits autophagic flux and induces cell death in human esophageal cancer cells through endoplasm reticulum stress-mediated Ca2+ release. Oncotarget. 2016 Jul; 7(30):47387-47402

 

Zheng K, Liao C, Li Y, Fan X, Fan L, Xu H, Kang Q, Zeng Y, Wu X, Wu H, Liu LZ, Xiao X, Zhang J, Wang Y, He Z. Gypenoside L, Isolated from Gynostemma pentaphyllum, Induces Cytoplasmic Vacuolation Death in Hepatocellular Carcinoma Cells through Reactive-Oxygen-Species-Mediated Unfolded Protein Response. J Agric Food Chem. 2016 Mar 2; 64(8): 1702-11.

 

Li MY, Liu Y, Liu LZ, Kong AW, Zhao Z, Wu B, Long X, Wu J, Ng CS, Wan IY, Du J, Mok TS, Underwood MJ, Chen GG.  Estrogen receptor alpha promotes smoking-carcinogen-induced lung carcinogenesis via cytochrome P450 1B1. J Mol Med (Berl). 2015 Nov; 93(11):1221-33.

 

Liu LZ, Cheung SC, Lan LL, Ho SK, Chan JC, Tong PC. Microtubule network is required for insulin-induced signal transduction and actin remodeling. Mol Cell Endocrinol. 2013 Jan 5;365(1):64-74.

 

Cheung SC, Long X, Liu LZ, Liu Q, Lan L, Tong PC, Sun SS. Inhibition of human MCF-7 breast cancer cells and HT-29 colon cancer cells by rice-produced recombinant human insulin-like growth binding protein-3 (rhIGFBP-3). PLoS One. 2013 Oct 15;8(10):e77516.

 

Zhao HL, Sui Y, Qiao CF, Yip KY, Leung RK, Tsui SK, Lee HM, Wong HK, Zhu X, Siu JJ, He L, Liu LZ, Xu HX, Tong PC and Chan JC. Sustained antidiabetic effects of a berberine-containing Chinese herbal medicine through regulation of hepatic gene expression. Diabetes, 2012, 61(4): 933-43.

 

Cheung SC*, Liu LZ*, Lan LL, Liu QQ, Sun SS, Chan JC and Tong PC. Glucose lowering effect of transgenic human insulin-like growth factor-I from rice: in vitro and in vivo studies. BMC Biotechnol. 2011, 11(1): 37-47 (*these authors contribute to this work equally)

 

Liu LZ, Cheung SC, Lan LL, Ho SK, Chan JC, Tong PC. The pivotal role of protein kinase C zeta (PKCzeta) in insulin- and AMP-activated protein kinase (AMPK) - mediated glucose uptake in muscle cells.  Cell. Signal. 2010, 22(10): 1513-22.

 

Liu LZ, Cheung SC, Lan LL, Ho SK, Xu HX, Chan JC, Tong PC. Berberine modulates insulin signaling transduction in insulin-resistant cells. Mol. Cell. Endocrinol. 2010, 317: 148-153

 

Zhao HL, Liu LZ, Sui Y, Ho SK, Tam SK, Lai FM, Chan JC, Tong PC. Fatty acids inhibit insulin-mediated glucose transport associated with actin remodeling in rat L6 muscle cells. Acta Diabetol. 2010 Dec;47(4):331-9. Acta Diabetol.

 

He A, Liu X, Liu LZ, Chang Y, Fang F. How many signals impinge on GLUT4 activation by insulin?  Cell. Signal. 2007, 19 (1): 1-7.

 

Yang C, Yang Y, Gupta N, Liu X, He A, Liu LZ, Zuo J, Chang Y, Fang F. Pentaspan membrane glycoprotein, prominin-1, is involved in glucose metabolism and cytoskeleton alteration. Biochemistry (Mosc). 2007, 72(8): 854-862

 

Liu LZ, Zhao HL, Zuo J, Ho SK, Chan JC, Meng Y, Fang FD, Tong PC. PKCzeta mediates insulin-induced glucose transport through actin remodeling in L6 muscle cells. Mol. Biol. Cell. 2006, 17:2322-2330.

 

Liu LZ, He AB, Liu XJ, Li Y, Chang YS, Fang FD. Protein Kinase C zeta and glucose uptake. Biochemistry (Mosc). 2006, 71(7): 701-706.

 

Li MY, Liu LZ, Huang SZ, Liu YP, Li H, Hu SY. Haptocyte growth factor suppress fibrogenic events in myofibroblastic MRC-5. Natl Med J China, 2002, 22(5): 422-427.

 

Liu JX, Liu L, Liu LZ, Su X, Liu SZ, Leng AJ. Cao Y.  Preparation of anti-PSP94 serum and analysis of human peripheral blood from normal persons and patients with benign prostate hyperplasia and prostate carcinoma. 2001, Med J Chin PLA, 26(1): 49-50

 

Liu QX, Liu L, Liu LZ, Xie BS, Wang Y, Leng AJ. The expression of fusion protein after injection of PSP94-TNFαD11a plasmid DNA in mice. Med J Chin PLA, 2001, 26(1): 60-61

 

Zhao XL, Liu L, Liu LZ, Shi Y, Xie BS, Wang Y, Yang Y, Leng AJ, Cao Y. Expression of shTNFR75 in yeast and bioassays of the expressed products. Chin J Microbiol. Immunol. 2001, 21(1): 99-100

 

Sun XW, Liu L, Shi Y, Liu LZ, Xie BS, Wang Y, Yang Y, Leng AJ, Cao Y. Construction and cell line selection of target-expressing retroviral expression vector containing cDNA of TNFR75. Chin. J. Biochem. Mol. Biol., 2001, 17(2): 169-172

 

Liu QX, Liu L, Shi Y, Liu LZ, Wang Y, Yang Y, Cao Y. Expression of PSP94-TNF αD11a fusion gene in NIH3T3 cells and bioassays of the expressed products. Chin. J. Biochem. Mol. Biol., 2001, 17(2): 194-197

 

Wang XH, Wang SQ, Guan W, Liu LZ, Mao BZ. The inhibitory effects of gene expression controlled by HCV 5′NCR in HepG2.9706 by hammerhead ribozyme. Chin. J. Biochem. Mol. Biol., 2001, 17(3): 391-394

 

Yan DL, Liu L, Shi Y, Liu LZ, Xie BS, Leng AJ, Cao Y. Effect of nuclear factor kappa B on the cytotoxity of TNF to prostate cancer. Chin J Urol. 2001, 22(3): 161-163

 

Yan DL, Liu L, Shi Y, Liu LZ, Xie BS. The role of NF-κB and TNF in resisting prostate cancer. Acta. Acad. Med. Shang Dong. 2001, 39(2): 145-148

Shi Y, Xiao C, Zhao XL, Wang Y, Liu L, Zhu X, Liu LZ, Xie BS, Leng AJ, Yang Y, Cao Y. Endothelial cell targeted expression of human tumor necrosis factor α mutant with higher activity from retroviral vectors. Immuno. J. 2001, 17(1): 30-33

 

Liu LZ, Xiao C, Liu L, Xie BS, Xu F, Leng AJ, Cao Y, Zhu X. et al. Gene Synthesis, Protein Expression and Function Identification of 502nd-764th Amino Acids in Extracellular Domain of KDR Human Vascular Endothelial Growth Factor Receptor. Chin. J. Biochem. Mol. Biol., 2000, 16(1): 86-91.

 

Liu LZ, Liu L, Xie BS, Leng AJ, Cao Y. The Expression of PSP94 mRNA In Prostate Carcinoma and Benign Prostatic Hyperplasia Detected by in Situ Hybridization. Med J Chin PLA, 2000, 25(2): 106-108.

 

Liu L, Liu LZ, Shi Y, Xie BS, Wang Y, leng AJ, Cao Y. Antitumor effect of PSP94-TNFαD11a fusion gene. Chin J Urol, 2000, 21(11): 676-678.

 

Liu L, Liu JX, Liu LZ, Shi Y, Xie BS, Leng AJ, Cao Y. Secretory Expression of Human PSP94 and the Antitumor Effect of the Expression Product on Prostate Carcinoma. Chin J Biochem. Mol. Biol., 2000, 164(4):542-545.

 

Liu L, Liu QX, Liu LZ, Shi Y, Xie BS, Leng AJ, Cao Y. Experiment Research on Human Prostate Carcinoma Therapy by combination PSP94 and TNFα Derivative. Chin J Biochem. Mol. Biol., 2000, 164(4):546-550.

 

Liu JX, Liu L, Liu LZ, Wang Y, Xie BS, Leng AJ, Su X, Liu SZ. Expression of Human Prostate Secretory Protein of 94 Amino Acids Fusion Protein in E.coli and Activity Assay of the Expressed Product. Prog. Biotech. 2000, 20(1): 8-12

 

Shi Y, Liu L, Liu LZ, Liu JX, Xie BS, Leng AJ. PSP57: A non-specific product of PSP94 mRNA variable splicing in prostatic tissues from patients with prostate carcinoma and benign prostatic hyperplasia. Acad J PLA Postgrad. Med. Sch. 2000, 21(3): 218-220

 

Liu LZ, Liu L, Xie BS, Leng AJ. The expressing of prostate secretory protein of 57 and 94 amino acids between normal and abnormal tissues. Med J Chin PLA, 1999, 24(6):495-497

 

Liu QX, Liu L, Liu JX, Liu LZ, Wang Y, Xie BS, Leng AJ. Human PSP94 cDNA obtained and expression of 5′PSP TNF Δ fusion protein in E.coli. Chin J Biochem. Mol.Biol. 1999, 15(3): 359-364

 

Liu L, Xu Q, Hu XM, Liu LZ, Wang Y, Xie BS, Leng AJ. Two 5′IL6 TNFα mutant fusion proteins with SAPGTP and PG linkers. Chin J Biochem. Mol.Biol. 1999, 15(4): 517-521

 

Liu Li, Liu JX, Liu QX, Liu LZ, Wang Y, Meng Y, Xie BS, Leng AJ, Su X, Liu SZ. mRNA expression of PSP mRNA in normal prostate tissue. Med J Chin PLA, 1999, 24(4): 265-267

 

Liu L, Wang Y, Tian SL, Lu XW, Meng Y, Liu LZ, Xie BS, Leng AJ. Studies on relations of thrombopoietin glycosylation and its biological effect in vivo. Immunol. J. 1999, 15(2): 103-105

 

Liu LZ, Wang SQ, Zhu BZ, Sun ZX. Study on cleavage of Hepatitis C virus RNA by specific ribozymes. Prog.  Biochem. Biophys. 1998, 25(5): 449-453.

 

Liu L, Liu LZ, Xie BS, Wang Y, Leng AJ. Separation and purification of the E.coli expressed rhTNFα D11α. Med J Chin PLA, 1998, 23(4):272-273.

 

Tian SL, Liu L, Lu XW, Meng Y, Xie BS, Liu LZ, Wang Y, Leng AJ. Secretion Expression and Activity Assay of Thrombopoietin in Pichia pastoris. Chin J Biochem. Mol. Biol. 1998, 14(4): 358-364

 

Tian SL, Liu L, Meng Y, Lu XW, Xie BS, Liu LZ, Leng AJ, Wang Y. Secretion expression and identification of thrombopoietin in Pichia pastoris. Chin J Microbiol. Immunol. 1998, 18(5): 397-400

 

Liu L, Tian SL, Wang Y, Lu XW, Meng Y, Xie BS, Liu LZ, Leng AJ. The Comparison between Biological Half life of TPO Mature Peptide Expressed in Piochia pastoris and TPO N terminal Domain Expressed in E.coli. Chin J Biochem. Mol. Biol. 1998, 14(6): 784-789

 

Liu L, Tian SL, Lu XW, Xie BS, Liu LZ, Wang Y. Stable expression and analysis of human TPO in CHO cells. Med J Chin PLA, 1998, 23(5):446-448.