BIOGRAPHICAL SKETCH 　 　

NAME: Xiong , Jixian 　 　

POSITION TITLE: Assistant Professor of Health Science Center , Shenzhen University School of Medicine 　 　

EDUCATION/TRAINING : 　 　

A.  Personal Statement 　 　

My long-standing research interests lie in cancer stem cells(CSCs), the m olecular   me chanisms of  c ancer ,   and developing targeted cancer drugs . Our work is mainly centered on   the identifi cation  and functional ly  characteriz ation of novel key molecule(s) involved in CSC  survival and self-renewal, and the elucidation of the molecular  mechanisms underlying tumor initiation, progression and metastasis. I have published 20  papers in professional peer-reviewed journals. 　 　 　

B.  Positions 　 　

Positions and Employment 　 　

01 / 2011 – 07 / 2014 Lecturer , Department of Biology , College of Science, Shantou University,   Shantou,  China 　 　

11 /201 4 – present         Assistant Professor , Department of medicine, Shenzhen University, Shenzhen, China 　 　 　

C.  Contributions to Science  (*: corresponding/co-corresponding author) 　 　

A full list of my publications is enclosed at the end of this document. 　 　

1.  Clusterin  in the survival and stemness  of gastric cancer stem cell 　 　

CSCs have been implicated in cancer relapse and metastasis ,  which may be  due to their capacity  for   uncontrolled growth, their resistance to chemo therapies and radiotherapies , and involvement in the Epithelial-Mesenchymal Transition. U nderstanding the weak spot of CSC s  and targeting this vulnerability will provide more effective treatment options and/or long-term cure s  for cancer patients .  In 20 17 , I   was the first to demonstrate that clusterin, a glycoprotein that acts in various biological  phenomena, is critical for Gastric Cancer Stem Cell ( GCSC )  survival  and self-renewal.  Further studies indicate d  that clusterin can be found in a complex with HSP90 and sustains GCSC survival by regulating Hsp90 function .  ( Under review, International Journal of Cancer ). 　 　

1)  Jixian Xiong ^# * , Shaoxiang Wang ^# , Tie Chen ^# , Xingsheng Shu ^# , Xianming Mo*, Gang Chang, Jia-Jie Chen   , Chenyang Li, Hui Luo, Jiing-Dwan Lee. Verteporfin blocks the expression of Clusterin which is required for survival of gastric cancer stem cell by modulating HSP90 function. International Journal of Cancer  ( Under review ) . 　 　

2.  Development of novel anti-cancer drug s 　 　

By screening a collection of drugs, we discovered  that a FDA-approved drug for purpose other than cancer therapy, verteporfin (VP), blocks   clusterin  expression , decreases HSP90 client proteins in GCSC and reduces the growth rate of GCSC xenograft ed tumor s  in animal  models . VP is currently in clinical use for conditions other than cancer. Re tasking this drug will , therefore, be rapid and cost-saving  as it is anticipated to overcome ( GCSC-mediated cancer metastasis, relapse and chemo-/radio-resistance )  that currently prevent effective and long-lasting cures for affected patients.   International Journal of Cancer   (Under review) . 　 　

1)  Jixian Xiong ^# * , Shaoxiang Wang ^# , Tie Chen ^# , Xingsheng Shu ^# , Xianming Mo*, Gang Chang, Jia-Jie Chen   , Chenyang Li, Hui Luo, Jiing-Dwan Lee. Verteporfin blocks the expression of Clusterin which is required for survival of gastric cancer stem cell by modulating HSP90 function. International Journal of Cancer  ( Under review ) .    

3.  T he   role and regulatory mechanism of Ras25 in gastric cancer 　 　

The function of Rab25 in cancer  has long been under debate, and  has gained increasing attention as a  potential   tumor therapy target  over recent   years. The clinical significance   of Rab25, especially the   prognostic significance, in   gastric cancer had not been   fully understood.   I demonstrated that  Rab25  has a pivotal role in tumor progression at   the tissue protein level and revealed the prognostic   significance of Rab25 expression in gastric   cancer, suggesting Rab25 to be a potential   therapy target of gastric cancer ( IJCEP 2017 ). 　 　

1)  Xiong  J * , Li  S , Zeng  X.  High Rab25 expression associates with Ki67/TP53/CD133/VEGFR expression predicts poor prognosis in gastric cancer. Int J Clin Exp Pathol ,  2017 , 10(7):7792-7800 .

D. Research Support 　 　

Ongoing Research Support 　 　

Natural Science Foundation of Guangdong Province    03 /20 1 8  to 03 / 2020 　 　

Xiong Jixian , PI           ￥ 3 0,000 (direct cost) 　 　

Research of effects of Clusterin on the survival, and stemness of   gastric cancer stem cell and its molecular mechanism s. 　 　

Completed Research Support 　 　

The Ph. D.   Programs Foundation of Ministry of Education of China     0 1 /01/20 14  to 12 /31/20 16 　 　

Xiong Jixian , PI                                       ￥ 4 0,000 　 　

The effects of Post-Translational Modifications on fatty acyl desaturase of Siganus canaliculatus 　 　

Natural Science Foundation of Guangdong Province  (S2013040014938) 10/01/2013 to 10/01/2015 　 　

Xiong Jixian , PI                                               ￥ 3 0,000 　 　

The effects of Post-Translational Modifications on the key enzymes of HUFA 　 　

Shenzhen Science and Technology Innovation Commission grant    0 8 / 31 /20 15  to 08 /3 1 /20 17 　 　

Xiong Jixian , PI                                 ￥ 30 0,000 　 　

Identification of tumor antigen peptides of gastric cancer stem cell and study of the peptides  inducing immunoeffects on anti-gastric cancer 　 　 　 　

E. Peer-reviewed publications (*: corresponding author) 　 　

1. Xiong   Jixian * ,  Li Shuiming ,  Zeng Xiandong . High Rab25 expression associates with   Ki67/TP53/CD133/VEGFR expression predicts poor prognosis in gastric cancer . International Journal of Clinical and Experimental Pathology , 2017, 10(7) : 7792-7800. 　 　

2. Zhou Jian ^# ,   Xiong Jixian ^# ,  Li Jianglin ,  Huang Sha ,  Zhang Hai ,  He Quanze ,  Lin Yong ,  Chen Ping ,  Wang Xianchun* ,  Liang Songping . Gel absorption-based sample preparation for the analysis of membrane proteome by mass spectrometry . Analytical Biochemistry, 2010, 404(2) : 204-210. 　 　

3.  Liu Yisong ,  Teng Xiaohua ,  Yang Xiaoxu ,  Song Qing ,  Lu Rong ,   Xiong Jixian ,  Liu Bo ,  Zeng Nianju ,  Zeng Yu ,  Long Jia ,  Cao Rui ,  Lin Yong ,  He Quanze ,  Chen Ping* ,  Lu Ming ,  Liang Songping . Shotgun Proteomics and Network Analysis Between Plasma Membrane and Extracellular Matrix Proteins From Rat Olfactory Ensheathing Cells . Cell Transplantation , 2010,  19(2) : 133-146. 　 　

4.  Li Xuanwen ,  Jin Qihui ,  Cao Jia ,  Xie Chunliang , Cao Rui ,  Liu Zhen ,   Xiong Jixian ,  Li Jianglin ,  Yang Xiaoxu ,  Chen Ping ,  Liang Songping* .  Evaluation of two cell surface modification methods for proteomic analysis of plasma membrane from isolated mouse hepatocytes . Biochimica ET Biophysica Acta-Proteins and Proteomics, 2009, 1794(1) : 32-41 . 　 　

5.  Xiong Xia ,  Huang Sha ,  Zhang Hai ,  Li Jianjun ,  Shen Jianying ,  Xiong Jixian ,  Lin Yong ,  Jiang Liping ,  Wang Xianchun* ,  Liang Sonping . Enrichment and proteomic analysis of plasma membrane from rat dorsal root ganglions . Proteome Science, 2009, 7 . 　 　

6.  Li Xuanwen ,  Cao   Jia ,  Jin Qihui ,  Xie Chunliang ,  He Quanyuan ,  Cao Rui ,   Xiong Jixian , Chen Ping ,  Wang Xianchun ,  Liang Songping* .  A proteomic study reveals the diversified distribution of plasma membrane-associated proteins in rat hepatocytes . Journal of Cellular Biochemistry, 2008, 104(3) : 965-984 . 　 　

7.  Zhang Lijun ,  Jia Xiaofang ,  Liu Xiaohui ,  Sheng Tingting ,  Cao Rui ,  He Quanyuan ,  Liu Zhen ,  Peng Xia , Xiong Jixian ,  Zhang Pengfei ,  Shi Ni ,  Liang Songping* . Dataset of the plasma membrane proteome of nasopharyngeal carcinoma cell line HNE1 for uncovering protein function . Acta Biochimica ET Biophysica Sinica, 2008, 40(1) : 55-70 . 　 　

8.  Deng EY ,  Liu YZ , Jiang C ,  Li LM ,  Wu S ,  Chen Y ,  Jiang H ,  Yang F ,  Xiao P ,  Xiao SM ,  Tan LJ ,  Sun X ,   Xiong JX ,  Zhu XZ ,  Liu MY , Lei SF ,  Chen XD ,  Xie JY ,  Xiao GG ,  Liang SP ,  Deng HW.   In vivo proteomic analysis of circulating monocytes in Chinese premenopausal females with extremely discordant bone mineral densit y. Proteomics, 2008, 8(20) : 4259-4272 . 　 　

9.  盛婷婷, 张丽军, 刘晓慧, 贾小芳, 熊继先,  贺权源, 曹锐, 彭霞, 石妮, 梁宋平. 不同分化程度的鼻咽癌细胞系质膜差异蛋白质组分析(英文). 中国生物化学与分子生物学报, 2008, 8:712-718. 　 　

10.  贾小芳, 石妮, 熊继先, 谢锦云, 梁宋平. 鼻咽癌细胞系与永生化的鼻咽上皮细胞系蛋白质表达差异分析(英文). 中国生物化学与分子生物学报, 2008, 01:11-19. 　 　

11.  Zhang Lijun ,  Wang Xi'e ,  Peng Xia ,  Wei Yingjuan ,  Cao Rui ,  Liu Zhen ,   Xiong Jixian ,  Ying Xingfeng ,  Chen Ping ,  Liang Songping* .  Immunoaffinity purification of plasma membrane with secondary antibody superparamagnetic beads for proteomic analysis . Journal of Proteome Research, 2007, 6(1) : 34-43 . 　 　

12.  Yuan Chunhua ,  Jin Qihui ,  Tang Xing ,  Hu Weijun ,  Cao Rui ,  Yang Shengqing ,   Xiong Jixian ,  Xie Chunliang ,  Xie Jinyun ,  Liang Songping* .  Proteomic and peptidomic characterization of the venom from the Chinese bird spider, Ornithoctonus huwena Wang . Journal of Proteome Research, 2007, 6(7) : 2792-2801 . 　 　

13.  陈平, 张丽军, 李选文, 王细娥, 曹锐, 刘珍, 熊继先 , 彭霞, 魏颖娟, 银兴峰, 王贤纯, 梁宋平. 鼠肝质膜蛋白质组研究的方法评估. 中国科学C辑:生命科学, 2007, 6:601-608. 　 　

14.  张丽军, 魏颖娟, 熊继先 , 贾小芳, 谢锦云, 梁宋平. 肝质膜蛋白质组研究中染色方法比较及优化. 湖南师范大学自然科学学报, 2007, 1:93-97. 　 　

15.  Chen P ,  Li X , Sun Y ,  Liu Z ,  Cao R ,   Xiong J ,  Liang S *.  Proteomic analysis of rat hippocampus plasma membrane: Characterization of potential neuronal-specific plasma membrane proteins . J Neurochem ,  2006 , 98(4):1126-40 . 　 　

16.  Zhang LJ ,  Wang X ,  Peng X ,  Wei YJ ,  Cao R ,  Liu Z ,   Xiong JX ,  Yin X F,  Ping C ,  Liang S*. Proteomic analysis of low-abundant integral plasma membrane proteins based on gels . Cellular and Molecular Life Sciences, 2006, 63(15) : 1790-1804 . 　 　

17.  Chen Ping ,  Li Xuanwen ,  Sun Yalan ,  Liu Zhen ,  Cao Rui ,  He Quanyuan ,  Wang Meichi ,   Xiong Jixian ,  Xie Jinyun ,  Wang Xianchun ,  Liang Songping* .  Proteomic analysis of rat hippocampal plasma membrane: characterization of potential neuronal-specific plasma membrane proteins . Journal of Neurochemistry,  2006,  98(4) : 1126-1140 . 　 　

18.  周铁杨, 周健, 熊继先 , 王贤纯. 基于等电聚焦-反相HPLC的虎纹捕鸟蛛毒素组学的初步研究. 中国生物化学与分子生物学报, 2006, 10:823-831. 　 　

19.  陈平, 李水明, 刘宇, 刘中华, 熊继先 , 孙亚兰, 曹锐, 梁宋平. 利用磺基异硫氰酸苯酯化学辅助方法鉴定磷酸化肽修饰位点. 中国生物化学与分子生物学报, 2006, 7:591-598. 　 　

20.  Duan ZG ,  Yan XJ ,  He XZ ,  Zhou H ,  Chen P ,  Cao R ,   Xiong JX ,  Hu WJ ,  Wang XC ,  Liang SP* .  Extraction and protein component analysis of venom from the dissected venom glands of Latrodectus tredecimguttatus . Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology,  2006,  145(3-4) : 350-357 .