NAME:BI, Wenchuan

POSITION TITLE:Assistant Professor, Shenzhen University School of Medicine

EDUCATION/TRAINING

A.   Personal Statement

My long-standing research interests fall in: 1) To reveal the molecular action mechanism of TCM. In my research, I have found that the underlying mechanisms of a Chinese herbal extract, Fo Shou San, in treating blood and vascular diseases.2) To investigate the P2Y Receptors in regulating cell proliferation, migration and neurogenesis of neuron stem cell. I have found that the P2y1 receptor could regulate the expression of acetylcholinesterase in muscle and neuron cells.

B.   Positions and Honors

Positions and Employment

0 3 /201 4 – 03/2017       Research Associate, Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong , China

0 6 /2017 – present      Assistant Professor , School of Pharmacy , Shenzhen University, Shenzhen, China

C.   Contributions to Science (*: corresponding/co-corresponding author)

A full list of my publications (in a total of 53) is enclosed at the end of this document.

The action mechanism of a TCM decoction Fo Shou San (FSS)

FSS is a Chinese medicinal formula for treating blood deficiency and cardiovascular diseases. Firstly, a systematic approach was developed for the chemical assessment of FSS. FSS was found to possess the ability to induce the red blood cell precursor differentiation. Also, FSS could induce erythropoietin (EPO) gene expression, which was mediated by hypoxia inducible factor (HIF) signaling pathway. We have also found that FSS possessed the ability to relax the rat coronary artery constriction, which was mediated by nitric oxide (NO). Furthermore, we have determined the absorption of ferulic acid of FSS using both in vitro and in vivo assay. For the in vitro assay, ferulic acid of FSS could be easily absorbed in Caco-2 intestinal cell monolayer; for the in vivo assay, the pharmacokinetic parameters showed that the bioavailability of ferulic acid of FSS was much higher than that ferulic acid alone after the oral administration to rats.

1)    Bi CW, Xu L, Tian XY, Liu J, Zheng KY, Lau CW, Lau DT, Choi RC, Dong TT, Huang Y, Tsim KW*. Fo Shou San, an ancient Chinese herbal decoction, protects endothelial function through increasing endothelial nitric oxide synthase activity. PLoS One. 2012; 7: e51670.

2)    Bi CW, Xie QH, Xu L, Li J, Cheung AW, Zheng KY, Chen VP, Lau DT, Choi RC, Wang TJ, Dong TT and Tsim KW*. Fo Shou San, an ancient herbal decoction prepared from Rhizoma Chuanxiong and Radix Angelicae Sinensis, stimulates the blood functions. Planta Med. 2010; 76: 1525-1529.

3)    Bi CW, Xu L, Zhang WL, Zhan JY, Fu Q, Zheng KY, Chen VP, Lau DT, Choi RC, Wang TJ, Dong TT and Tsim KW*. Fo Shou San, an ancient herbal decoction prepared from Angelicae Sinensis Radix and Chuanxiong Rhizoma induces erythropoietin expression: a signaling mediated by the reduced degradation of hypoxia-inducible factor in cultured liver cells. Planta Med. 2012; 78: 122-127.

D.   Research Support

Ongoing Research Support

Shenzhen Science and Technology Innovation Commission grant (JCYJ201708183000926)                                                                                                                 04/01/2018 to 03/31/2020

Completed Research Support

NSFC (China) (81403087)                                          01/01/2015 to 12/31/2017

BI, Wenchuan, PI                                                               ￥230,000

E.    Peer-reviewed publications (*: corresponding author)

1. Cui JD, Xu ML, Liu EY, Dong TT, Lin HQ, Tsim KW, Bi CW*. Expression of globular form acetylcholinesterase is not altered in P2Y1R knock-out mouse brain. Chem Biol Interact. 2016;25:291-294.
2. Miernisha A^#, Bi CW^#, Cheng LK, Xing JG, Liu J, Maiwulanjiang M, Aisa HA, Dong TT, Lin H, Huang Y, Tsim KW*. Badiranji Buya Keli, a Traditional Uyghur Medicine, Induces Vasodilation in Rat Artery: Signaling Mediated by Nitric Oxide Production in Endothelial Cells.Phytother Res. 2015; 10.
3. Maiwulanjiang M^#, Bi CW^#, Lee PS, Xin G, Miernisha A, Lau KM, Xiong A, Li N, Dong TT, Aisa HA, Tsim KW*. The volatile oil of Nardostachyos Radix et Rhizoma induces endothelial nitric oxide synthase activity in HUVEC cells. PLoS One. 2015; 10: e0116761.
4. Bi CW , Xu L, Gong AG, Dong TT, Zhang TH, Li Q, Tsim KW*.Pharmacokinetic analyses of ferulic acid in rat plasma by liquid chromatography–tandem mass spectrometry: A synergistic action of an ancient herbal decoction Fo Shou San. Pharm Anal Acta. 2015, 6: 5.
5. Bi CW , Luk WK, Campanari ML, Liu YH, Xu L, Lau KM, Xu ML, Choi RC, Sáez-Valero J, Tsim KW*. Quantification of the transcripts encoding different forms of AChE in various cell types: real-time PCR coupled with standards in revealing the copy number. J Mol Neurosci. 2014; 53: 461-468.
6. Bi CW , Xu L, Tian XY, Liu J, Zheng KY, Lau CW, Lau DT, Choi RC, Dong TT, Huang Y, Tsim KW*. Fo Shou San, an ancient Chinese herbal decoction, protects endothelial function through increasing endothelial nitric oxide synthase activity. PLoS One. 2012; 7: e51670.
7. Bi CW , Xie QH, Xu L, Li J, Cheung AW, Zheng KY, Chen VP, Lau DT, Choi RC, Wang TJ, Dong TT and Tsim KW*. Fo Shou San, an ancient herbal decoction prepared from Rhizoma Chuanxiong and Radix Angelicae Sinensis, stimulates the blood functions. Planta Med. 2010; 76: 1525-1529.
8. Bi CW , Xu L, Zhang WL, Zhan JY, Fu Q, Zheng KY, Chen VP, Lau DT, Choi RC, Wang TJ, Dong TT and Tsim KW*. Fo Shou San, an ancient herbal decoction prepared from Angelicae Sinensis Radix and Chuanxiong Rhizoma induces erythropoietin expression: a signaling mediated by the reduced degradation of hypoxia-inducible factor in cultured liver cells.Planta Med.2012; 78: 122-127.
9. Xu M, Luk WK, Bi CW, Liu EY, Wu KQ, Yao P, Dong TT, Tsim KW.Erythropoietin regulates the expression of dimeric form of acetylcholinesterase during differentiation of erythroblast.J Neurochem. 2018 Apr 20. [Epub ahead of print].
10. Liu R, Li P, Bi CW, Ma R, Yin Y, Bi K, Li Q.Plasma N-acetylputrescine, cadaverine and 1,3-diaminopropane: potential biomarkers of lung cancer used to evaluate the efficacy of anticancer drugs.Oncotarget. 2017;8:88575-88585.
11. Yan L, Wei M, Gong AG, Song P, Lou J, Bi CW, Xu SL, Xiong A, Dong TT, Tsim KW.A Modified Chinese Herbal Decoction (Kai-Xin-San) Promotes NGF-Induced Neuronal Differentiation in PC12 Cells via Up-Regulating Trk A Signaling.Front Cell Dev Biol. 2017; 22: 118.
12. Lou JS, Yan L, Bi CW, Chan GK, Wu QY, Liu YL, Huang Y, Yao P, Du CY, Dong TT, Tsim KW.Yu Ping Feng San reverses cisplatin-induced multi-drug resistance in lung cancer cells via regulating drug transporters and p62/TRAF6 signalling.Sci Rep. 2016;6:31926.
13. Xu H, Liu R, He B, Bi CW, Bi K, Li Q.Polyamine Metabolites Profiling for Characterization of Lung and Liver Cancer Using an LC-Tandem MS Method with Multiple Statistical Data Mining Strategies: Discovering Potential Cancer Biomarkers in Human Plasma and Urine.Molecules. 2016;21: 8.
14. Xu L, Chen Y, Xie HQ, Xu T, Fu H, Zhang S, Tsim KW, Bi CW, Zhao B. 2,3,7,8-Tetrachlorodibenzo-p-dioxin suppress AChE activity in NGF treated PC12 cells.Chem Biol Interact. 2016.[Epub ahead of print]
15. Yan L, Hu Q, Mak MS, Lou J, Xu SL, Bi CW, Zhu Y, Wang H, Dong TT, Tsim KW.A Chinese herbal decoction, reformulated from Kai-Xin-San, relieves the depression-like symptoms in stressed rats and induces neurogenesis in cultured neurons. Sci Rep. 2016;6:30014.
16. Xiong A, Yan AL, Bi CW, Lam KY, Chan GK, Lau KK, Dong TT, Lin H, Yang L, Wang Z, Tsim KW*.Clivorine, an otonecine pyrrolizidine alkaloid from Ligularia species, impairs neuronal differentiation via NGF-induced signaling pathway in cultured PC12 cells.Phytomedicine. 2016; 23: 931-938.
17. Lau KM, Gong AG, Xu ML, Lam CT, Zhang LM, Bi CW, Cui D, Cheng AW, Dong TT, Tsim KW, Lin H*.Transcriptional activity of acetylcholinesterase gene is regulated by DNA methylation during C2C12 myogenesis. Brain Res. 2016; 1642: 114-123.
18. Xu ML, Bi CW, Kong AY, Dong TT, Wong YH, Tsim KW*.Flavonoids induce the expression of acetylcholinesterase in cultured osteoblasts.Chem Biol Interact. 2016. [Epub ahead of print].
19. Xu ML, Bi CW, Cheng LK, Mak S, Yao P, Luk WK, Lau KK, Cheng AW, Tsim KW*. Reduced expression of P2Y2 receptor and acetylcholinesterase at neuromuscular junction of P2Y1 receptor knock-out mice. J Mol Neurosci. 2015; 57: 446–451.
20. Xu ML, Luk WK, Lau KK, Bi CW, Cheng AW, Gong AG, Lin H, Tsim KW*. Three N-glycosylation sites of human acetylcholinesterase shares similar glycan composition. J Mol Neurosci. 2015; 57: 486-491.
21. Liu R, Ma R, Yu C, Bi CW, Yin Y, Xu H, Shang H, Bi K, Li Q*. Quantitation of eleven active compounds of Aidi injection in ratplasma and its application to comparative pharmacokinetic study. Journal of Chromatography B. 2016; 1026: 105-113.
22. Du CY, Zheng KY, Bi CW, Dong TT, Lin H, Tsim KW*. Yu Ping Feng San, an ancient Chinese herbal decoction, induces gene expression of anti-viral proteins and inhibits neuraminidase activity. Phytother Res. 2015;29:656-661.
23. Chen J, Lam CT, Kong AY, Zhang WL, Zhan JY, Bi CW, Chan GK, Lam KY, Yao P, Dong TT, Tsim KW*. The extract of Ziziphus jujuba fruit (Jujube) induces expression of erythropoietin via hypoxia-Inducible factor-1α in cultured Hep3B cells. Planta Med. 2014; 80: 1622-1627.
24. Zhan JY, Yao P, Bi CW, Zheng KY, Zhang WL, Chen JP, Dong TT, Su ZR, Tsim KW*. The sulfur-fumigation reduces chemical composition and biological properties of Angelicae Sinensis Radix. Phytomedicine. 2014; 21: 1318-1324.
25. Ding Y, Yang Z, Bi CW, Yang M, Xu SL, Lu X, Huang N, Huang P, Leng Y*. Directing vascular cell selectivity and hemocompatibility on patterned platforms featuring variable topographic geometry and size. ACS Appl Mater Interfaces. 2014; 13: 12062-70.
26. Ding Y, Yang Z, Bi CW, Yang M, Zhang J, Xu SL, Lu X, Huang N, Huang P, Leng Y*. Modification of protein adsorption, vascular cell selectivity and platelet adhesion by mussel-inspired surface functionalization. J. Mater. Chem. B. 2014; 2: 3819.
27. Zhan JY, Zheng KY, Zhang WL, Chen JP, Yao P, Bi CW, Dong TT, Tsim KW*. Identification of Angelica oil as a suppressor for the biological properties of Danggui Buxue Tang: a Chinese herbal decoction composes of Astragali Radix and Angelica Sinensis Radix. J Ethnopharmacol. 2014; 154: 825-831.
28. Zheng KY, Zhang ZX, Du CY, Zhang WL, Bi CW, Choi RC, Dong TT, Tsim KW*. Ferulic acid enhances the chemical and biological properties of Astragali Radix: a stimulator for Danggui Buxue Tang, an ancient Chinese herbal decoction. Planta Med. 2014; 80: 159-164.
29. Xu SL, Zhu KY, Bi CW, Yan L, Men SW, Dong TT, Tsim KW*. Flavonoids, derived from traditional Chinese medicines, show roles in the differentiation of neurons: possible targets in developing health food products. Birth Defects Res C Embryo Today. 2013; 99: 292-299.
30. Xu SL, Zhu KY, Bi CW, Choi RC, Miernisha A, Yan AL, Maiwulanjiang M, Men SW, Dong TT, Tsim KW*. Flavonoids induce the expression of synaptic proteins, synaptotagmin, and postsynaptic density protein-95 in cultured rat cortical neuron. Planta Med. 2013; 79: 1710-1714.
31. Xu SL, Bi CW, Choi RC, Zhu KY, Miernisha A, Dong TT, Tsim KW*. Flavonoids induce the synthesis and secretion of neurotrophic factors in cultured rat astrocytes: a signaling response mediated by estrogen receptor. Evid Based Complement Alternat Med. 2013; 2013:127075.
32. Zhang WL, Zheng KY, Zhu KY, Zhan JY, Bi CW, Chen JP, Dong TT, Choi RC, Lau DT, Tsim KW*. Chemical and biological assessment of Angelica roots from different cultivated regions in a Chinese herbal decoction Danggui Buxue Tang. Evid Based Complement Alternat Med. 2013; 2013: 483286.
33. Zhan JY, Zheng KY, Zhu KY, Zhang WL, Bi CW, Chen JP, Du CY, Dong TT, Lau DT, Tsim KW*. Importance of wine-treated Angelica Sinensis Radix in Si Wu Tang, a traditional herbal formula for treating women's ailments. Planta Med. 2013; 79: 533-537.
34. Zhang WL, Zheng KY, Zhu KY, Zhan JY, Bi CW, Chen JP, Du CY, Zhao KJ, Lau DT, Dong TT, Tsim KW*. Chemical and biological assessment of Angelica herbal decoction: comparison of different preparations during historical applications. Phytomedicine. 2012; 19: 1042-1048.
35. Zheng KY, Choi RC, Guo AJ, Bi CW, Zhu KY, Du CY, Zhang ZX, Lau DT, Dong TT, Tsim KW*. The membrane permeability of Astragali Radix-derived formononetin and calycosin is increased by Angelicae Sinensis Radix in Caco-2 cells: a synergistic action of an ancient herbal decoction Danggui Buxue Tang. J Pharm Biomed Anal. 2012; 70: 671-679.
36. Zheng KY, Zhang ZX, Guo AJ, Bi CW, Zhu KY, Xu SL, Zhan JY, Lau DT, Dong TT, Choi RC, Tsim KW*. Salidroside stimulates the accumulation of HIF-1α protein resulted in the induction of EPO expression: a signaling via blocking the degradation pathway in kidney and liver cells. Eur J Pharmacol. 2012; 679: 34-39.
37. Wang T, Choi RC, Li J, Bi CW, Ran W, Chen X, Dong TT, Bi K, Tsim KW*. Trillin, a steroidal saponin isolated from the rhizomes of Dioscorea nipponica, exerts protective effects against hyperlipidemia and oxidative stress. J Ethnopharmacol. 2012; 139: 214-220.
38. Zhan JY, Zheng KY, Zhu KY, Bi CW, Zhang WL, Du CY, Fu Q, Dong TT, Choi RC, Tsim KW, Lau DT*. Chemical and biological assessment of Angelicae Sinensis Radix after processing with wine: an orthogonal array design to reveal the optimized conditions. J Agric Food Chem. 2011; 59: 6091-6098.
39. Zheng KY, Choi RC, Cheung AW, Guo AJ, Bi CW, Zhu KY, Fu Q, Du Y, Zhang WL, Zhan JY, Duan R, Lau DT, Dong TT, Tsim KW*. Flavonoids from Radix Astragali induce the expression of erythropoietin in cultured cells: a signaling mediated via the accumulation of hypoxia-inducible factor-1α. J Agric Food Chem. 2011; 59: 1697-1704.
40. Zheng KY, Guo AJ, Bi CW, Zhu KY, Chan GK, Fu Q, Xu SL, Zhan JY, Lau DT, Dong TT, Choi RC, Tsim KW*. The extract of Rhodiolae Crenulatae Radix et Rhizoma induces the accumulation of HIF-1α via blocking the degradation pathway in cultured kidney fibroblasts. Planta Med. 2011; 77: 894-899.
41. Zheng KY, Choi RC, Xie HQ, Cheung AW, Guo AJ, Leung KW, Chen VP, Bi CW, Zhu KY, Chan GK, Fu Q, Lau DT, Dong TT, Zhao KJ, Tsim KW*. The expression of erythropoietin triggered by Danggui Buxue Tang, a Chinese herbal decoction prepared from Radix Astragali and Radix Angelicae Sinensis, is mediated by the hypoxia-inducible factor in cultured HEK293T cells. J Ethnopharmacol. 2010; 132: 259-267.
42. Wang T, Choi RC, Li J, Li J, Bi CW, Zang L, Liu Z, Dong TT, Bi K, Tsim KW*. Antihyperlipidemic effect of protodioscin, an active ingredient isolated from the rhizomes of Dioscorea nipponica. Planta Med. 2010; 76: 1642-1646.
43. Guo AJ, Xie HQ, Choi RC, Zheng KY, Bi CW, Xu SL, Dong TT, Tsim KW*. Galangin, a flavonol derived from Rhizoma Alpiniae Officinarum, inhibits acetylcholinesterase activity in vitro. Chem Biol Interact. 2010; 187: 246-248.
44. Xie HQ, Leung KW, Chen VP, Chan GK, Xu SL, Guo AJ, Zhu KY, Zheng KY, Bi CW, Zhan JY, Chan WK, Choi RC, Tsim KW*. PRiMA directs a restricted localization of tetrameric AChE at synapses. Chem Biol Interact. 2010; 187: 78-83.
45. Zheng YZ, Choi RC, Li J, Xie HQ, Cheung AW, Duan R, Guo AJ, Zhu JT, Chen VP, Bi CW, Zhu Y, Lau DD, Dong TT, Lau BW, Tsim KW*. Ligustilide suppresses the biological properties of Danggui Buxue Tang: a Chinese herbal decoction composed of Radix Astragali and Radix Angelica Sinensis. Planta Med. 2010; 76: 439-443.
46. Tsim KW*, Leung KW, Mok KW, Chen VP, Zhu KY, Zhu JT, Guo AJ, Bi CW, Zheng KY, Lau DT, Xie HQ, Choi RC. Expression and Localization of PRiMA-linked globular form acetylcholinesterase in vertebrate neuromuscular junctions. J Mol Neurosci. 2010; 40: 40-46.
47. Zhu JT, Choi RC, Xie HQ, Zheng KY, Guo AJ, Bi CW, Lau DT, Li J, Dong TT, Lau BW, Chen JJ, Tsim KW*. Hibifolin, a flavonol glycoside, prevents beta-amyloid-induced neurotoxicity in cultured cortical neurons. Neurosci Lett. 2009; 461: 172-176.
48. Zhu JT, Choi RC, Li J, Xie HQ, Bi CW, Cheung AW, Dong TT, Jiang ZY, Chen JJ, Tsim KW*. Estrogenic and neuroprotective properties of scutellarin from Erigeron breviscapus: a drug against postmenopausal symptoms and Alzheimer's disease. Planta Med. 2009; 75: 1489-1493.
49. Cheung JK, Li J, Cheung AW, Zhu Y, Zheng KY, Bi CW, Duan R, Choi RC, Lau DT, Dong TT, Lau BW, Tsim KW*. Cordysinocan, a polysaccharide isolated from cultured Cordyceps, activates immune responses in cultured T-lymphocytes and macrophages: signaling cascade and induction of cytokines. J Ethnopharmacol. 2009; 124: 61-68.
50. Li WZ, Li J, Bi CW, Cheung AW, Huang W, Duan R, Choi RC, Chen IS, Zhao KJ, Dong TT, Duan JA,Tsim KW*. Can Rhizoma Chuanxiong replace Radix Angelica Sinensis in the traditional Chinese herbal decoction Danggui Buxue Tang? Planta Med. 2009; 75: 602-606.
51. Li J, Li WZ, Huang W, Cheung AW, Bi CW, Duan R, Guo AJ, Dong TT, Tsim KW*. Quality evaluation of Rhizoma Belamcandae (Belamcanda chinensis (L.) DC.) by using high-performance liquid chromatography coupled with diode array detector and mass spectrometry. J Chromatogr A. 2009; 1216: 2071-2078.
52. Lau FT, Choi RC, Xie HQ, Leung KW, Chen VP, Zhu JT, Bi CW, Chu GK, Tsim KW*. Myocyte enhancer factor 2 mediates acetylcholine-induced expression of acetylcholinesterase-associated collagen ColQ in cultured myotubes. Mol Cell Neurosci. 2008; 39: 429-438.
53. Tsim KW*, Choi RC, Xie HQ, Zhu JT, Leung KW, Lau FT, Chu GK, Chen VP, Mok MK, Cheung AW, Bi CW. Transcriptional control of different subunits of AChE in muscles: signals triggered by the motor nerve-derived factors. Chem Biol Interact. 2008; 175: 58-63.